维氏气单胞菌flrB基因敲除株构建及其生物特性鉴定

维氏气单胞菌是一种常见的人鱼共患致病菌，为研究维氏气单胞菌中的flrB基因的功能，笔者通过扩增flrB基因上下游同源臂连接至pRE112质粒中，电击转化重组质粒至大肠杆菌WM3064，接合转入野生型维氏气单胞菌，在蔗糖压力下筛选、PCR和测序验证敲除菌株。结果表明，敲除flrB基因后降低生物膜的形成，但不影响维氏气单胞菌的生长。flrB基因能促进其生物膜的形成，这为进一步揭示该基因的功能和作用机制奠定了基础。

Construction of a C4 flrB Knockout Strain of Aeromonas veronii and its Biological Characteristics

Aeromonas veronii is a common merman comorbid pathogen. To study the function of the flrB gene in Aeromonas veronii, the upstream and downstream homology arms of the flrB gene were amplified and integrated to the pRE112 plasmid, and the recombinant plasmid was transformed into E. coli WM3064 by electroporation, and then was conjugated into the wild-type A. veronii. After screened under sucrose pressure, the knockout strains were verified by PCR and sequencing. The flrB gene knockout strain is tested for growth curve and biofilm formation. The biofilm test showed that the knockout of flrB reduced the formation of biofilm, but did not affect the growth of A. veronii. This revealed that the flrB gene could promote biofilm formation of A. veronii, which lays a foundation for further revealing the function and mechanism of this gene.