| 日本囊对虾apo-14基因的全克隆及序列分析 |
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| 引用本文： | 乔莹,王军,钟声平,刘洪涛,毛勇.日本囊对虾apo-14基因的全克隆及序列分析[J].热带生物学报,2013,4(1):17-24. |
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| 作者姓名： | 乔莹 王军 钟声平 刘洪涛 毛勇 |
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| 作者单位： | 厦门大学海洋与地球学院,福建厦门,361005 |
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| 基金项目： | 国家虾产业技术体系岗位专家项目：“日本囊对虾育种”（CARS-47） |
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| 摘 要： | 以日本囊对虾(Marsupenaeus japonicus)肝胰腺组织为实验材料,提取总RNA,通过3′以及5 '-RACE基因克隆技术,首次在日本囊对虾中克隆到载脂蛋白apolipoprotein-14(apo-14)基因的全长序列,包含完整的ORF以及3′-UTR和5′-UTR.日本囊对虾apo-14 cDNA序列全长为784 bp,其中ORF长度为420 bp,编码139个氨基酸.对虾apo-14氨基酸序列与其他鱼类apo-14氨基酸序列有较高的相似性.
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| 关 键 词： | 日本囊对虾 载脂蛋白 apo-14 克隆 序列分析 |
Cloning and Sequence Analysis of the apo-14 Gene in Marsupenaeus japonicus |
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| QIAO Ying
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WANG Jun
,
ZHONG Shengping
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LIU Hongtao
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MAO Yong.Cloning and Sequence Analysis of the apo-14 Gene in Marsupenaeus japonicus[J].Journal of Tropical Biology,2013,4(1):17-24. |
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| Authors: | QIAO Ying WANG Jun ZHONG Shengping LIU Hongtao MAO Yong |
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| Institution: | ( College of Ocean and Earth Sciences,Xiamen University,Xiamen 361005,China) |
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| Abstract: | Full length of apolipoprotein-14 （apo-14） gene was cloned from the total RNA of Marsupenaeus japonicus. Sequence analysis showed that the full cDNA sequence was 784 bp in length including the coding region of 420 bp and encoded 139 amino acids. Homology analysis of the apolipoprotein-14 amino acid sequence indicated high sequence similarity in apo-14 amino acid sequences between M. japonicus and other fish. |
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| Keywords: | Marsupenaeus japonicas apolipoprotein apo-14 cloning sequence analysis |
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