| 病毒抑制剂对蝴蝶兰病毒植株的脱毒效果 |
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| 引用本文： | 李正民,王安石,王健,陶楚.病毒抑制剂对蝴蝶兰病毒植株的脱毒效果[J].热带生物学报,2013,4(1):56-60. |
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| 作者姓名： | 李正民 王安石 王健 陶楚 |
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| 作者单位： | 1. 海南大学园艺园林学院,海南海口,570228
2. 海南出入境检验检疫局热带植物隔离检疫中心,海南海口,570311 |
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| 基金项目： | 三亚市院地合作项目“蝴蝶兰脱毒苗工厂化繁育技术研究”（2010YD59） |
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| 摘 要： | 选取感染建兰花叶病毒(CyMV)和齿兰环斑病毒(ORSV)的蝴蝶兰植株进行离体培养,在芽增殖培养基中添加不同的病毒抑制剂,研究对CyMV和ORSV病毒的脱除效果.结果表明,氨基寡糖素具有很好的脱毒效果,对CyMV的脱除率为83.33％,对ORSV的脱除率为66.67％；病毒唑次之,对CyMV的脱除率为83.33％,对ORSV的脱除率为50％；中药类的脱毒效果不明显.
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| 关 键 词： | 蝴蝶兰 脱毒 CyMV ORSV |
Detoxification Effect of Virus Inhibitors on Viruses in Phalaenopsis |
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| LI Zhengmin
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WANG Anshi
,
WANG Jian
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TAO Chu.Detoxification Effect of Virus Inhibitors on Viruses in Phalaenopsis[J].Journal of Tropical Biology,2013,4(1):56-60. |
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| Authors: | LI Zhengmin WANG Anshi WANG Jian TAO Chu |
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| Institution: | 1 ( 1. College of Horticulture and Landscape,Hainan University,Haikou 570228,China; 2. Post-Entry Quarantine Station for Tropical Plants,Hainan Entry-Exit Inspection and Quarantine Bureau,Haikou 570311,China) |
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| Abstract: | Cymbidium mosaic virus （ CyMV） and Odontoglossum ringspot virus （ ORSV） are the most serious viruses which affect the quality of Phalaenopsis plants. It is of a great significance to control CyMV and ORSV. Proliferation buds of Phalaenopsis inoculated with both the CyMV and ORSV viruses were used as explants and cultured on proliferation medium added with different virus inhibitors. The result showed that amino-oligosaccharin gave the highest CyMV and ORSV elimination rate,up to 83. 33% and 66. 67% ,respectively,followed by ribavirin with the respective elimination rates of 83. 33% （ CyMV） and 50% （ ORSV） . The Chinese Herbs inhibitor was the worst. |
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| Keywords: | Phalaenopsis virus-free CyMV ORSV |
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