湛江东海岛对虾育苗场水体细菌的抗生素耐药性

利用K-B纸片扩散法研究分离自湛江东海岛6个对虾育苗场(分别编号为YF,JD,DC,HM,SD,XR)水体中的217株细菌菌株对12种抗生素的耐药性。研究结果表明,参试的12种抗生素之间的细菌耐药性差异显著(ANOVA,P0.05),6个育苗场内细菌对多粘菌素B、左氟沙星和环丙沙星高度敏感,对氨苄西林、甲氧苄胺嘧啶、复方新诺明和四环素则高度耐药;多重耐药菌株在每个育苗场中均有发现,52.07%的菌株呈现多重耐药性,未发现对参试的12种抗生素100%耐药的菌株。统计分析结果显示,尽管HM和XR育苗场的细菌耐药性比其他育苗场高,但6个对虾育苗场之间水体细菌的抗生素耐药性无显著差异(ANOVA,P0.05)。

Antibiotic Resistance of Bacteria Isolated from Shrimp Hatcheries at Donghai Island,Zhanjiang

At Donghai Island, Zhanjiang, Guangdong, China were isolated 217 strains of bacteria from the water in 6 shrimp hatcheries, and their antibiotic resistance and susceptibility to 12 antibiotic agents was determined by using the Kirby-Bauer disc diffusion method for susceptibility testing. The results showed significant differences in bacterial resistance of the water to the 12 antibiotics （ ANOVA, P 〈 0.05 ）. Bacteria of the water in the 6 hatcheries showed high susceptibility to polymyxin-B, levofloxacin, and ciprofloxacin and gave high resistance to ampicillin, trimethoprim, compound sinomi and tetracycline. Bacterial strains with multiple antibiotic resistances （MAR） were found in the water from each hatchery, and about 52.07% of strains exhibited MAR, but none of the strains showed resistance to all 12 antibiotics. Statistical analysis indicated that no significant difference in antibiotic resistance of bacteria was observed in the water among the 6 hatcheries （ ANOVA, P = 0.273 〉 0.05 ） although antibiotic resistance of bacteria isolated from the water were higher in the hatcheries HM and XR than in the other hatcheries.