| 靶向bcr/abl融合基因的RNAi对慢性粒细胞白血病K562增殖活性的影响 |
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| 引用本文： | 潘彦鹏,;曹献英,;武昭颖,;常志杰,;王银银,;田博,;郝新宝.靶向bcr/abl融合基因的RNAi对慢性粒细胞白血病K562增殖活性的影响[J].热带生物学报,2014(1):25-29. |
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| 作者姓名： | 潘彦鹏 ;曹献英 ;武昭颖 ;常志杰 ;王银银 ;田博 ;郝新宝 |
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| 作者单位： | [1]海南大学材料与化工学院,海南海口570228; [2]清华大学医学院/生物膜与膜生物工程国家重点实验室,北京100084; [3]陕西中医学院附属医院肿瘤外科,陕西咸阳712083; [4]海南医学院附属医院,海南海口570228 |
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| 摘 要： | 构建含bcr/abl RNAi慢病毒重组质粒载体并包装病毒,转染K562细胞,通过Real-time PCR及Western blotting验证干扰效应。采用CCK-8法、软琼脂细胞集落形成实验检测RNAi对K562细胞的增殖影响作用。结果表明,慢病毒介导bcr/abl基因RNAi明显下调K562细胞中bcr/abl mRNA及P210bcr/abl融合蛋白,显著抑制了K562细胞的增殖,这说明靶向bcr/abl融合基因的RNAi有望成为治疗慢性粒细胞白血病的有效方法。
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| 关 键 词： | 慢病毒 bcr/abl基因 Pbcr/abl 增殖 |
The Effect of RNAi Targeting bcr/abl Fusion Gene on the Proliferation Activity of the Chronic Myelogenous Leukemia K562 |
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| Institution: | PAN Yanpeng , CAO Xianying , WU Zhaoying, CHANG Zhijie, WANG Yinyin, TIAN Bo, HAO Xinbao ( 1. College of Materials Sciences and Chemical Engineering, Hainan University, Haikou 570228, China; 2. School of Medicine/State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China; 3. Department of Surgical Oncology, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712083, China; 4. Affiliated Hospital of Hainan Medical College, Haikou 570228, China) |
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| Abstract: | bcr/abl RNAi lentivirus vector was constructed and used to transfect K562 cells （the first human immortalised myelogenous leukemia line to be established）,and the stable transfectants were selected.RNAi efficiency was assessed by Real-time PCR and Western blotting.The viability of the K562 cells was detected by CCK-8 assay and clonogenic assay.The results showed that bcr/abl RNAi significantly down regulates the amount of bcr/abl mRNA and P210bcr/abl fusion protein,and inhibits the proliferation of the K562 cells.The method of RNAi targeting bcr/abl fusion gene highlights a new way of chronic myelogenous leukemia treatment. |
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| Keywords: | lentivirus bcr/abl gene P210bcr/abl proliferation |
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