决明子多糖的IR指纹图谱甄别技术

以决明子为研究对象,对其多糖进行了红外分析,建立了江西决明子多糖的红外指纹图谱标准化共有模式(相似度0.935~0.998)。基于该模式,分析发现未知决明子多糖的相似度仅为0.782,进而提取11个共有峰,通过对共有峰的主成分分析和聚类分析,表明未知决明子和江西决明子属于不同类别。该方法可以作为基于多糖类功效成分的原料的质量控制手段。

Discrimination of Cassia Seed Polysaccharides by Fingerprint Infrared Spectroscopy

The polysaccharides from cassia seeds （seeds of Cassia obtusifolia or C.tora） were analyzed by using fingerprint infrared spectrometry.A model for infrared fingerprint spectra of polysaceharides from 10 batches of cassia seeds collected in Jiangxi was established and standardized with a similarity ranging from 0.935 to 0.998.With this model the cassia seeds polysaccharide from unknown source was evaluated,and the similarity was merely 0.782.Furthermore,11 co-existing peaks were chosen from the IR fingerprints of the polysaccharides from 10 batches of cassia seeds collected in Jiangxi and resorted to principal component analysis and hierarchical cluster analysis.The results showed that the cassia seeds from the unknown source was different from those collected in Jiangxi and was in a different category.The fingerprinting method and principle component analysis could be applied to supervise the quality of the products or raw materials that have bioactive components of polysaeeharides.