广东省花生青枯病病原的分离和遗传多样性鉴定

为了解广东省花生青枯病病原菌的遗传多样性，对广东省主栽花生品种近年来发生青枯病的病株进行病原菌分离，共获得64株分离菌株，其中31株为青枯菌（Ralstonia solanacearum）．遗传多样性分析，发现31株青枯菌菌株均为演化型I型．随机挑取不同分离地点的花生青枯菌菌株进行致病力测定，发现来自不同地区的青枯菌致病力有所不同，其中来自湛江市的ZKRS126菌株致病力明显具有材料特异性，对花生A300致病力强而对花生A281致病力弱．此外，进化树分析的亲缘关系较近菌株致病力也有明显差异，近缘菌株ZKRS126与ZKRS206对花生A300的致病力具有显著性差异．本研究不仅有助于广东省花生青枯病的防治与研究，而且为抗青枯病花生品种的选育和研究提供基础数据和依托材料．

Isolation and genetic diversity identification of peanut bacterial wilt pathogen in Guangdong Province

In order to understand the genetic diversity of the pathogen of peanut bacterial wilt in Guangdong Province, 64 strains were isolated from wilt peanut plants of main planting in Guangdong Province in recent years, among which 31 were Ralstonia solanacearum strains. Genetic diversity analysis of the 31 R. solanacearum strains demonstrated that they all belonged to phylotype I. The R. solanacearum strains from different places were randomly selected for pathogenicity analysis. The result showed that the pathogenicity of these strains was different. Among them, the pathogenicity of strain ZKRS126 from Zhanjiang was obviously variety specific. It showed strong pathogenicity to peanut A300 but weak pathogenicity to peanut A281. In addition, the pathogenicity of strains with close genetic relationship was also significantly different. The pathogenicity of ZKRS126 and ZKRS206 to peanut A300 was significantly different. This study will contribute to the control and research of peanut bacterial wilt in Guangdong Province, and provide basic data and supporting materials for the subsequent research and breeding of resistant peanut varieties.