| 高产复合酶益生菌的氮离子注入诱变选育 |
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| 作者姓名： | 陈丽娟 张云华 王宝玉 孟秀丽 程茂基 |
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| 作者单位： | 安徽农业大学动物科技学院 合肥230006(陈丽娟，孟秀丽，程茂基)，安徽农业大学生命科学学院 合肥230006(张云华，王宝玉) |
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| 基金项目： | 国家自然科学基金项目“利用低能离子束生物工程技术选育益生菌及其机理研究”（30100134）,安徽农业大学校长青年基金项目“复合酶提高松针粉饲用效果的应用研究”（060863）. |
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| 摘 要： | 以产复合酶益生菌黑曲霉ANO1为出发菌株,经多次N 注入诱变处理,结合平板透明圈法定向选育,得到高产变异菌株ANO2。结果显示,出发菌株ANO1酸性蛋白酶、纤维素酶和果胶酶的酶活显著提高,分别由71.6IU/g、141.7IU/g和264.8IU/g提高到996.5IU/g、940.4IU/g和906.5IU/g。变异菌株ANO2经传5代培养,产酶特性稳定。同时对原菌株和变异菌株的生物学特性进行了相关的研究,结果表明变异菌株的生物量和产酶量成正相关。
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| 关 键 词： | N 注入 黑曲霉 诱变选育 生物学特性 |
Breeding of High-yield Compound Enzyme Probiotic Aspergillus niger by N+ Implantation |
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| Authors: | Chen Lijuan Zhang Yunhu Wang Baoyu Meng Xiuli Cheng Maoji |
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| Institution: | 1College of Animal Science, Anhui Agriculture University, Hefei 230036; 2College of Life Science, Anhui Agriculture University, Hefei 230036 |
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| Abstract: | A mutated strain ANO2 with high yield of compound enzymes was obtained from the strain ANO1 of Aspergillus niger by N implantation, and screened by using transparent zones of agar plate screening techniques. The results showed that activities of acid-protease, Cellulase and Pectinase in Aspergillus niger were raised signifcantly from 71.6 IU/g, 141.7 IU/g and 264.8 IU/g to 996.5 IU/g, 940.4 IU/g and 906.5 IU/g respectively. Characteristics of enzymes production in mutated strain Aspergillus niger AN02 were stabile by 5 times subculture.Biological characteristics of the original strain ANO1 and the mutant strain ANO2 were researched. Biomass and enzyme activity of the mutant strain ANO2 were increasing relativity. |
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| Keywords: | N implantation Aspergillus niger Mutant breeding Biological characteristics |
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