| 基于冠心病血瘀证心肌细胞能量代谢网络模型的流平衡分析 |
| |
| 作者姓名： | 袁肇凯 简维雄 黄献平 张月娟 孙安会 夏世靖 |
| |
| 作者单位： | 湖南中医药大学中医诊断学国家重点学科, 湖南 长沙 410007;国家中医药管理局局级重点实验室, 湖南 长沙 410208,湖南中医药大学中医诊断学国家重点学科, 湖南 长沙 410007;国家中医药管理局局级重点实验室, 湖南 长沙 410208,湖南中医药大学中医诊断学国家重点学科, 湖南 长沙 410007;湖南中医药大学湖南省重点实验室, 湖南 长沙 410208,湖南中医药大学中医诊断学国家重点学科, 湖南 长沙 410007,湖南中医药大学中医诊断学国家重点学科, 湖南 长沙 410007,湖南中医药大学中医诊断学国家重点学科, 湖南 长沙 410007 |
| |
| 基金项目： | 国家自然科学基金项目（81273670；81202647）；教育部博士点基金项目（20124323130001）。 |
| |
| 摘 要： | 目的 探讨冠心病（coronary artery heart disease，CHD）急、慢性血瘀证心肌细胞能量代谢的变化特点。方法 （1）运用FluxExplorer软件平台构建起的CHD血瘀证心肌细胞能量代谢网络模型；（2）应用GC-MS技术和血气自动分析仪从健康对照、CHD慢性血瘀证、CHD急性血瘀证三组大鼠模型中取冠状动脉血检测葡萄糖、脂肪酸及血氧含量；（3）检测结果代入网络模型，通过流平衡分析CHD急、慢性血瘀证状态下参数的特点。结果 （1）三组的糖酵解、脂代谢和三羧酸循环均已被激活，但网络运算后各组代谢物的数值则有明显差别，均呈现出健康对照组>CHD慢瘀证组>CHD急瘀证组的趋势。（2）三组心肌细胞能量代谢的血糖、脂肪酸和血氧含量及ATP的产量均呈递减的趋势，这与三组中糖酵解的关键酶（HK、pfk、PK）、三羧酸循环的关键酶（Cs、Icdh、Od）的浓度呈一致性变化。结论 CHD急性血瘀和慢性血瘀不同证候的形成可能与机体内心肌细胞能量代谢的水平密切相关。
|
| 关 键 词： | 冠心病 血瘀证 能量代谢 网络 流平衡分析 心肌细胞 |
| 收稿时间： | 2015/10/21 0:00:00 |
The Flux Balance Analysis based on Network Model of Myocardial Cell Energy Metabolism of Blood Stasis Syndrome in Coronary Heart Disease |
| |
| Authors: | YUAN Zhaokai JIAN Weixiong HUANG Xianping ZHANG Yuejuan SUN Anhui and XIA Shijing |
| |
| Institution: | Key Discipline of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Key Laboratory of State Administration of Traditional Chinese Medicine Administration, Changsha, Hunan 410208, China,Key Discipline of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Key Laboratory of State Administration of Traditional Chinese Medicine Administration, Changsha, Hunan 410208, China,Key Discipline of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Hunan Key Labtory of Hunan University Of Chinese Medicine, Changsha, Hunan 410208, China,Key Discipline of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China,Key Discipline of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China and Key Discipline of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China |
| |
| Abstract: | Objective To investigate the characteristics of myocardial cell energy metabolism changes of acute and chronic blood stasis syndrome of coronary heart disease (CHD). Methods (1) The CHD myocardial cell energy metabolism network model of blood stasis syndrome were built up by using FluxExplorer software platform. (2) The coronary arterial blood glucose, fatty acids and blood oxygen content, which were taken from from healthy control group, CHD with chronic blood stasis syndrome, CHD with acute blood stasis syndrome groups, were detemined by GC-MS technology and automatic blood gas analyzer. (3) The results were into network model, the characteristic parameters of acute and chronic CHD with blood stasis syndrome were analyzed by flux balance. Results (1) Three groups of glycolytic, lipid metabolism and the TCA cycle has been activated, but after the network operations metabolite values were significantly different in each group, which showed a healthy control group >CHD with chronic blood stasis syndrome >CHD with acute blood stasis group trend. (2) The glucose, fatty acids and blood oxygenation content and yield of ATP in three groups showed a decreasing trend. The key enzymes of glycolysis (HK, PFK, PK), key enzymes of the TCA cycle (Cs, Icdh, Od) showed consistent changes. Conclusion The form of different CHD with acute blood stasis and chronic blood stasis syndromes were closely related to levels of myocardial energy metabolism in the body. |
| |
| Keywords: | coronary heart disease blood stasis syndrome energy metabolism networking flux balance analysis myocardial cell |
|
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |
|
