| 短短芽孢杆菌JK-2菌株抑茵活性物质产生条件的优化 |
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| 引用本文： | 郝晓娟,刘波,葛慈斌,周先治.短短芽孢杆菌JK-2菌株抑茵活性物质产生条件的优化[J].河北农业科学,2009,13(6):46-49. |
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| 作者姓名： | 郝晓娟 刘波 葛慈斌 周先治 |
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| 作者单位： | 1. 山西农业大学农学院,山西,太谷,030801
2. 福建省农业科学院生物技术研究所,福建,福州,350003 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，山西农业大学博士科研启动基金 |
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| 摘 要： | 短短芽孢杆菌JK-2菌株对多种植物病原真菌和病原细菌具有明显的抑制作用。以JK．2发酵液对番茄枯萎病菌的抑制作用为活性指标，对其培养基成分和培养条件进行了优化。由正交试验和单因素试验得出的最佳培养基配方为淀粉1，0％、牛肉浸膏0．5％、蛋白胨0．3％、蔗糖1．0％、酵母粉0．5％和CaCl2 0．5％，初始pH值7．0。在30℃、170r／min振荡培养条件下，最佳发酵时间为48h，装液量100mL／瓶，接种量1％。
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| 关 键 词： | 短短芽孢杆菌 抑菌物质 拮抗细菌 培养条件 优化 |
Optimization of Culture Conditions of Brevibacillus brevis Strain JK-2 for Producing Antibacterial Substances |
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| HAO Xiao-juan,LIU Bo,GE Ci-bin,ZHOU Xian-zhi.Optimization of Culture Conditions of Brevibacillus brevis Strain JK-2 for Producing Antibacterial Substances[J].Journal of Hebei Agricultural Sciences,2009,13(6):46-49. |
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| Authors: | HAO Xiao-juan LIU Bo GE Ci-bin ZHOU Xian-zhi |
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| Institution: | 1.College of Agriculture;Shanxi Agricultural University;Taigu 030801;China;2.Biotechnology Institute;Fujian Academy of Agricultural Sciences;Fuzhou 350003;China |
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| Abstract: | Brevibacillus brevis strain JK-2 could produce antibacterial substances which significantly inhibited various plant pathogenic fungi and bacteria.Using the inhibition effect of JK-2 fermentation broth on Fusarium oxysporum as the activity index,the medium components and culture conditions were optimized.The optimum medium components were obtained by orthogonal experiment and single factor experiment,which included 1.0% starch,0.5% beef extract,0.3% peptone,1.0% sucrose,0.5% yeast extract and 0.5% CaCl2,and ... |
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| Keywords: | Brevibacillus brevis Antibacterial substance Antagonistic bacteria Culture conditions Optimization |
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