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养殖水体耐盐高效降亚硝酸盐氮和氨氮芽孢杆菌的筛选与鉴定
引用本文:高海英,王占武,李洪涛,张翠绵,田洪涛,贾楠.养殖水体耐盐高效降亚硝酸盐氮和氨氮芽孢杆菌的筛选与鉴定[J].河北农业科学,2008,12(11).
作者姓名:高海英  王占武  李洪涛  张翠绵  田洪涛  贾楠
作者单位:1. 河北省农林科学院遗传生理研究所,河北,石家庄,050051;河北农业大学,河北,保定,071000
2. 河北省农林科学院遗传生理研究所,河北,石家庄,050051
3. 河北农业大学,河北,保定,071000
摘    要:亚硝酸盐氮和氨氮是养殖水体恶化的主要成分。从对虾养殖水体中,分离筛选出2株分别对亚硝酸盐氮和氨氮具有较高降解能力的耐盐芽孢杆菌菌株T905和T301。在模拟淡水和海水条件下,当亚硝酸盐氮和氨氮初始浓度分别为44 mg/L和20 mg/L时,3 d后菌株T905对亚硝酸盐氮降解率分别达到72.10%和92.10%,T301对氨氮降解率分别达到55.18%和52.00%。根据形态学特征和生理生化试验结果,鉴定2株菌为枯草芽孢杆菌。

关 键 词:养殖水体净化  亚硝酸盐氮  氨氮  分离  鉴定  芽孢杆菌

Screening and Identification of the Salt-tolerance and High-effective Bacillus Removing Nitrite Nitrogen and Ammonia Nitrogen in Aquaculture Water
GAO Hai-ying,WANG Zhan-wu,LI Hong-tao,ZHANG Cui-mian,TIAN Hong-tao,JIA Nan.Screening and Identification of the Salt-tolerance and High-effective Bacillus Removing Nitrite Nitrogen and Ammonia Nitrogen in Aquaculture Water[J].Journal of Hebei Agricultural Sciences,2008,12(11).
Authors:GAO Hai-ying  WANG Zhan-wu  LI Hong-tao  ZHANG Cui-mian  TIAN Hong-tao  JIA Nan
Institution:GAO Hai-ying1,2,WANG Zhan-wu1*,LI Hong-tao1,ZHANG Cui-mian1,TIAN Hong-tao2,JIA Nan1
Abstract:Nitrite nitrogen and ammonia nitrogen were the main components in the deteriorative aquaculture water.Salt-tolerance and high-effective Bacillus strains T905 and T301 removing nitrite nitrogen and ammonia nitrogen respectsively were isolated from shrimp aquaculture water.In simulation fresh water and sea aquaculture water containing nitrite nitrogen 44 mg/L and amomonia nitrogen 20 mg/L,after 3 days,nitrite nitrogen was degraded 72.10% and 92.10% respectively by T905,and ammonia nitrogen was degraded 55.18% and 52.00% respectively by T301.According to morphology characteristics and the results of physiological and biochemical experiments,the two strains were identified as Bacillus subtilis.
Keywords:Aquaculture water purification  Nitrite nitrogen  Ammonia nitrogen  Isolation  Identification  Bacillus
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