| 变异焊菜组织培养及无性系建立的研究 |
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| 引用本文： | 孙立业,张卓,全俊龙,佟少明,姜长阳.变异焊菜组织培养及无性系建立的研究[J].河北农业科学,2007,11(5):14-16,38. |
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| 作者姓名： | 孙立业 张卓 全俊龙 佟少明 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁,大连,116029 |
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| 摘 要： | 以野生变异焊菜根上部的生长点为外植体,以附加不同浓度的BA、IAA、NAA、2,4-D、GA的MS、1/2MS或1/3MS为培养基,进行了芽伸长生长培养、芽分化培养和试管苗生根培养研究.结果表明:1/2 MS IAA 0.2 mg/L是促进变异焊菜培养芽伸长生长的理想培养基,MS BA 0.5 mg/L NAA 0.1 mg/L GA 0.5 mg/L是变异焊菜芽分化培养的理想培养基,1/3 MS IAA 0.5 mg/L是变异焊菜试管苗生根培养的理想培养基;变异焊菜试管苗移植后,有利的变异性状保持不变.
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| 关 键 词： | 焊菜 组织培养 愈伤组织 无性系 |
| 文章编号： | 1008-1631（2007）05-0014-03 |
| 修稿时间： | 2007-07-31 |
Study on Tissue Culture and Asexuality Establishment of Variation of Rorippa indica (L.) Hiem |
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| SUN Li-ye,ZHANG Zhuo,QUAN Jun-long,TONG Shao-ming,JIANG Chang-yang.Study on Tissue Culture and Asexuality Establishment of Variation of Rorippa indica (L.) Hiem[J].Journal of Hebei Agricultural Sciences,2007,11(5):14-16,38. |
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| Authors: | SUN Li-ye ZHANG Zhuo QUAN Jun-long TONG Shao-ming JIANG Chang-yang |
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| Institution: | College of Life Science, Liaoning Normal University, Dalian 116029, China |
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| Abstract: | Corms of saffron crocus(Crocus sativus) were used as explants and cultured in the dark on MS medium supplemented with BA,IAA,NAA,24-D or GA.The results showed that the oppotimum medium to promote bud growth,bud regeneration and root regeneration was 1/2 MS supplemented with IAA 0.2 mg/L;MS containing BA 0.5 mg/L,NAA 0.1 mg/L and GA 0.5 mg/L,1/3 MS containing IAA 0.5 mg/L,The transferred cormlets could remain the advantageous variation characteristics. |
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| Keywords: | Rorippa indica(L ) Hiem Tissue culture Callus Clone |
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