| 百萨偃麦草高分子量麦谷蛋白亚基的鉴定及其染色体定位 |
| |
| 引用本文： | 李辉,李义文,陈豫,贾旭.百萨偃麦草高分子量麦谷蛋白亚基的鉴定及其染色体定位[J].河北农业科学,2006,10(3):1-5. |
| |
| 作者姓名： | 李辉 李义文 陈豫 贾旭 |
| |
| 作者单位： | 1. 河北省农林科学院粮油作物研究所,河北省作物遗传育种实验室,河北,石家庄,050031;中国科学院遗传与发育生物学研究所,国家植物细胞与染色体工程重点实验室,北京,100101
2. 中国科学院遗传与发育生物学研究所,国家植物细胞与染色体工程重点实验室,北京,100101 |
| |
| 摘 要： | 经SDS-PAGE电泳和Western杂交,在百萨偃麦草(Thinopyrum bessarabicum)中鉴定出1个高分子量麦谷蛋白亚基,其电泳迁移率大于"中国春"小麦的1Dy12亚基,本研究命名为1Ebx亚基,其编码基因命名为Glu-1Ebx.对由百萨偃麦草衍生的双二倍体及其1套二体附加系进行SDS-PAGE分析,只有百萨偃麦草、双二倍体和1Eb附加系表达1Ebx亚基,小麦亲本及其它附加系均不表达此亚基.用1对HMW-GS通用引物对上述材料进行扩增时,只有在百萨偃麦草、双二倍体和1Eb染色体附加系的基因组DNA中扩增出分子量大小一致的DNA片段,与SDS-PAGE的定位结果一致.表明1Ebx亚基是由百萨偃麦草1Eb染色体上的HMW-GS基因所编码.
|
| 关 键 词： | 百萨偃麦草 高分子量麦谷蛋白亚基 染色体 定位 |
| 文章编号： | 1008-1631(2006)03-0001-05 |
| 收稿时间： | 2006-05-29 |
| 修稿时间： | 2006年5月29日 |
Identification and Chromosomal Location of a High Molecular Weight Glutenin Subunit from Thinopyrum Bessarabicum |
| |
| LI Hui,LI Yi-wen,CHEN Yu,JIA Xu.Identification and Chromosomal Location of a High Molecular Weight Glutenin Subunit from Thinopyrum Bessarabicum[J].Journal of Hebei Agricultural Sciences,2006,10(3):1-5. |
| |
| Authors: | LI Hui LI Yi-wen CHEN Yu JIA Xu |
| |
| Institution: | 1. Key Laboratory of Crop Genetics and Breeding of Hebei Province, Institute of Food and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031 , China; 2. The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology Chinese Academy of Sciences, Beijing 100101, China |
| |
| Abstract: | A high molecular weight glutenin subunit(HMW-GS) which has a faster electrophoretic mobility than that of 1Dy12 subunit from the bread wheat Chinese Spring,was identified from Thinopyrum bessarabicum by SDS-PAGE and Western Blotting,designated as 1E~bx in this study.In order to determine the precise location of 1E~bx subunit,SDS-PAGE analysis was carried out in amphidiploid and additional lines deduced from Thinopyrum bessarabicum.Results of SDS-PAGE revealed that 1E~bx subunit was specially expressed in Thinopyrum bessarabicum,amphidiploid and 1E~b additional lines,other than wheat parent lines and other additional lines.Consistence with the location identified by SDS-PAGE,a band with the similar molecular weight was also amplified only in Thinopyrum bessarabicum,amphidiploids and 1E~b additional lines using a pair of degenerate primers of HMW-GS.These results showed that the 1E~bx subunit was encoded by a HMW-GS gene,Glu-1E~bx,on chromosome 1E~b of Thinopyrum bessarabicum. |
| |
| Keywords: | Thinopyrum bessarabicum High molecular weight glutenin subunit(HMW-GS) Chromosome location |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
