| 细果角茴香中总黄酮的提取及含量测定 |
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| 引用本文： | 刘永红,陈晓,汶凯,彭措卓玛,梁永欣.细果角茴香中总黄酮的提取及含量测定[J].河北农业科学,2011,15(6):106-108. |
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| 作者姓名： | 刘永红 陈晓 汶凯 彭措卓玛 梁永欣 |
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| 作者单位： | 青海民族大学化学与生命科学学院,青海西宁,810007 |
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| 基金项目： | 青海省重点科技项目(2010-Z-719) |
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| 摘 要： | 利用热回流法和超声波法提取细果角茴香中的总黄酮，结合NaNO2-Al（NO3）3-NaOH体系分光光度法测定细果角茴香中的总黄酮含量，并对细果角茴香中总黄酮的提取方法进行优化。结果表明：利用超声波法提取细果角茴香中的总黄酮效果较好；且在甲醇浓度为70％、料液比为1：20、超声波提取2次、提取时间分别为2．0h和1．5h的条件下提取效果最好，测得总黄酮的含量为15．44mg／g。NaNO2-Al（NO3）3-NaOH体系分光光度法操作简捷，测定结果准确可靠。
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| 关 键 词： | 细果角茴香 总黄酮 提取 含量测定 |
Extraction and Content Determination of Total Flavonoids in Hypecoum leptocarpum Hook.f.et Thoms. |
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| LIU Yong-hong,CHEN Xiao,WEN Kai,PENGCUO Zhuo-ma,LIANG Yong-xin.Extraction and Content Determination of Total Flavonoids in Hypecoum leptocarpum Hook.f.et Thoms.[J].Journal of Hebei Agricultural Sciences,2011,15(6):106-108. |
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| Authors: | LIU Yong-hong CHEN Xiao WEN Kai PENGCUO Zhuo-ma LIANG Yong-xin |
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| Institution: | LIU Yong-hong,CHEN Xiao,WEN Kai,PENGCUO Zhuo-ma,LIANG Yong-xin(College of Chemistry and Life Science,Qinghai University for Nationalities,Xining 810007,China) |
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| Abstract: | Total flavonoids in H. leptocarpum was extracted by hot reflux method and ultrasonic extraction method, and its content was determined by NaNO2-Al （NO3 ）3-NaOH spectrometry. The results showed that ultrasonic method was more suitable than hot reflux method, and the optimum extraction condition was 70% methanol solution, solid-liquid ratio 1：20 （W/V）, ultrasonic treatment twice with the time of 2. 0 h and 1.5 h. Total flavonoids content in H. leptocarpum was about 15.44 mg/g. NaNO2-Al （NO3 ） 3-NaOH spectrometry method was easy to operate and the results were accurate. |
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| Keywords: | Hypecoum leptocarpum Hook f et Thoms Total flavonoids Extraction Content determination |
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