SRAP标记技术在谷子及其近缘野生种青狗尾草的应用分析

青狗尾草（Setaria viridis）被认为是谷子（S.italica）的祖先种,因此研究青狗尾草与谷子品种间的亲缘关系,对于野生有益基因的发掘利用有着深远意义。以来源于不同国家的谷子和青狗尾草共24个品种为试材,分析SRAP技术在谷子和青狗尾草中的适用性研究。结果表明：（1）用24个标记组合共产生127条多态性带,平均每个标记组合产生5.29条多态扩增带。每对引物组合产生的多态性带的比例为16.67%～37.90%,平均为28.30%。说明供试青狗尾草和谷子具有较为丰富的遗传多样性。（2）对所有材料进行聚类分析,遗传相似系数为0.56～0.90,平均遗传相似系数为0.79。相似系数为0.56时,可以将24份材料分成2大类群：第一类群全部为青狗尾草,第二类群则包括谷子和部分青狗尾草材料。综上所述,SRAP技术能够有效地对谷子和青狗尾草属进行多态性分析。

Application of SRAP Technology on Foxtail Millet and Its Wild Relative Green Foxtail

Wild green foxtail（Setaria viridis） was regarded as the ancestor of foxtail millet（S.italica）.The phynogentic relationship between green foxtail and foxtail millet varieties was of profound significance.24 accessions of foxtail millet and green foxtail were analyzed by SRAP technology.The results showed that 127 polymorphic bands were amplified with 24 pairs of primers.The average proportion of polymorphic band with each pair of primers was 28.30%,that was to say,green foxtail and foxtail millet were rich in genetic diversity.The genetic similarity coefficients among each accession ranged from 0.56 to 0.90 with the average of 0.79.Cluster analysis revealed that 24 accessions were divided into two groups,one group included green foxtail varieties,and the other included part of green foxtail varieties and foxtail millet.