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杀伤细胞免疫球蛋白样受体在输血中的作用
引用本文:吴兆勇,郑丽,周君纯.杀伤细胞免疫球蛋白样受体在输血中的作用[J].湛江医学院学报,2011,29(1):20-23.
作者姓名:吴兆勇  郑丽  周君纯
作者单位:吴兆勇,周君纯,WU Zhao-yong,ZHOU Jun-chun(广东医学院第二附属医院输血科,广东湛江,524003);郑丽,ZHENG Li(中山大学附属第六医院检验科,广东广州,510655)
摘    要:目的 初步探讨杀伤细胞免疫球蛋白样受体在输血中的作用.方法 用免疫磁珠法分离正常人外周血NK细胞,再用PCR-SSP方法进行KIR分型.将分型的NK细胞与已知HLA分型的全血混合,37℃培养0、6、12、24h,ELISA法检测IL-2、IL-4、前列腺素E2(PGE2)、TNF-α、TNF-β、IFN-γ、粒细胞-巨...

关 键 词:杀伤细胞免疫球蛋白样受体  输血

Role of killer cell immunoglobulin-like receptor in blood transfusion
WU Zhao-yong,ZHENG Li,ZHOU Jun-chun.Role of killer cell immunoglobulin-like receptor in blood transfusion[J].Journal of Guangdong Medical College,2011,29(1):20-23.
Authors:WU Zhao-yong  ZHENG Li  ZHOU Jun-chun
Institution:1.Department of Blood Transfusion,the Second Affiliated Hospital of Guangdong Medical College,Zhanjiang 524003,China)
Abstract:Objective To study the role of killer cell immunoglobulin-like receptor in blood transfusion.Methods KIR genotypes of NK cells were determined by PCR-SSP after isolated from peripheral blood of healthy subjects using immuno-magnetic beads.NK cells were then mixed with the whole blood with known HLA typing,and cultured at 37 ℃ for 0,6,12,24 h.Levels of IL-2,IL-4,PGE2,TNF-α,TNF-β,IFN-γ,GM-CSF,IgA,IgM and IgG were detected by ELISA.Results Levels of IL-2,IL-4,PGE2,NF-α,TNF-β,IFN-γ and GM-CSF increased,but those of IgA,IgM and IgG decreased in a time-dependent manner(P0.05).Along with the inconsistent degree of KIR and HLA(0%,20%,40%,60% and 80%),contents of TNF-α,TNF-β,IFN-γ and GM-CSF were upregulated(P0.05),but those of IL-2,IL-4,PGE2,IgA,IgM and IgG showed unchangeable.Conclusion The immunologic function of allogeneic NK cells can be induced by the mismatch of KIR and HLA-I,leading to the reversal of transfusion related immune inhibition.
Keywords:killer cell immunoglobulin-like receptor  blood transfusion
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