| 氯沙坦对过氧化氢诱导乳鼠心肌细胞凋亡的保护作用机制研究 |
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| 引用本文： | 刘文东,李悦山.氯沙坦对过氧化氢诱导乳鼠心肌细胞凋亡的保护作用机制研究[J].湛江医学院学报,2011,29(6):591-595. |
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| 作者姓名： | 刘文东 李悦山 |
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| 作者单位： | 刘文东 (广州医学院药理学教研室,广东广州,510182) ； 李悦山 (广州医学院药理学教研室,广东广州,510182) ； |
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| 摘 要： | 目的 探讨氯沙坦对过氧化氢诱导心肌细胞凋亡的保护作用机制.方法 在原代培养SD乳鼠心肌细胞上建立H2O2损伤模型,分别以低、中、高剂量(1、3、10μmol/L)氯沙坦、10μmol/L氯沙坦+50μmol/L LY294002处理,检测各组心肌细胞存活率及丙二醛(MDA)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD...
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| 关 键 词： | 氯沙坦 过氧化氢 心肌细胞 |
Protective mechanism of losartan on hydrogen peroxide-induced cardiocytc apoptosis in suckling rats |
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| LIU Wen-dong,LI Yue-shan.Protective mechanism of losartan on hydrogen peroxide-induced cardiocytc apoptosis in suckling rats[J].Journal of Guangdong Medical College,2011,29(6):591-595. |
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| Authors: | LIU Wen-dong LI Yue-shan |
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| Institution: | (Department of Pharmacology, Guangzhou Medical College, Guangzhou 510182, China) |
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| Abstract: | Ojective To investigate the protective mechanism of losartan on hydrogen peroxide-induced cardioeyte apoptos- is in suckling rats. Methods Primary cultured cardiocytes of suckling rats were used to establish H202-induced apoptosis model, and then treated with 1, 3, and 10 ~ mol/L losartan, and 10 1μ mol/L losartan plus 50 μ mol/L LY294002, respectively. Cardiocyte viability and levels of malonaldehyde （MDA）, lactate dehydrogenase （LDH）, superoxide dismutase （SOD）, caspase3/7 and p-Akt were measured. Results Compared with H2O2 group and LY294002 group, cardioeyte viability and expression of LDH, SOD and P-Akt were upregulated, while that of MDA and easpase3/7 was downregulated in losartan group. Conclusion Losartan can inhibit H202-induced cardiocyte apoptosis in suckling rats via stabilizing cytomembrane, resisting lipid peroxidation, clearing oxygen free radical and activating PI3K/Akt pathway. |
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| Keywords: | losartan hydrogen peroxide cardiocyte |
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