肝毒蠲汤抗大鼠四氯化碳肝损伤的实验研究

目的 :观察肝毒蠲汤对四氯化碳 (CCl4 )所致大鼠急性肝损伤的防治作用。方法 :48只大鼠随机分为 6组 :正常对照组 ,肝损伤模型组 ,联苯双酯组和肝毒蠲低、中、高剂量组。除正常对照组外 ,其余各组用 CCl4 造成大鼠急性实验性肝损伤 ;模型组给予生理盐水 ,其余 4组分别给予联苯双酯和肝毒蠲低、中、高剂量 ,共治疗 14 d。实验结束后 ,检测各组大鼠的血清丙氨酸转氨酶 (AL T)、天门冬氨酸转氨酶 (AST)含量 ,并取肝脏作病理组织检查。结果 :肝毒蠲高、中、低剂量组血清 ALT、AST水平均较模型组明显降低 (P<0 .0 0 1) ,且肝毒蠲高剂量组降酶作用较低剂量组明显 (P<0 .0 0 1)。经肝毒蠲处理组大鼠肝组织基本结构未发生明显改变 ,肝细胞脂肪变性及炎症细胞浸润均较模型组明显减少。结论 :肝毒蠲能显著降低 CCl4 所致急性肝损伤大鼠血清 ALT、AST水平 ,对 CCl4 所致大鼠的肝损伤具有显著的防治作用。

Effects of Gandujuan decoction on carbon tetrachloride-induced liver injury in rats

Objective: To observe the protective and curative effects of Gandujuan decoction on acute liver damage induced by carbon tetrachloride(CCl 4) in rats.Methods:Fourty-eight rats were randomly divided into six groups: control group, model group, bifendate group, and high, moderate and low doses of Gandujuan decoction groups. Except control group, liver injury of rats in other groups was induced by CCl 4 and the rats were simultaneously treated by bifendate, and three different doses of Gandujuan decoction for 14 days, respectively. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and liver pathological changes were detected. Results: ALT and AST levels were significantly lower in Gandujuan decoction groups than those in model group (P<0.001), especially in high dose of Gandujuan decoction group. Compared with model group, no obvious changes of liver morphology, and less fatty degeneration and inflammatory cell infiltration were observed in Gandujuan decoction group. Conclusion: Gandujuan decoction treatment can remarkably reduce serum levels of ALT and AST, and provide protective effects on hepatic damage caused by CCl 4 in rats.