间接ELISA法检测人血清抗HBcIgA及其应用

目的：改良抗ＨＢｃＩｇＡ的检测方法，并探讨抗ＨＢｃＩｇＡ与肝脏损伤程度的关系，方法：通过预测处理患者血清，以羊抗人ＩｇＡ－ＨＲＰ（ＳＡＨ－ＩｇＡ－ＨＲＰ）为指示系统，建立间接ＥＬＩＳＡ法，并对２８０例临床血清进行检测。结果：经与抗体捕获酶联夹心法相比较，两种方法所检测出的抗ＨＢｓＩｇＡ阳性率之间及Ｓ／Ｎ值之间的差异均我显著性，在２８０例临床血清标本中，重症乙型肝炎，慢性活动性肝炎（简称慢活肝）合并

DETECTION AND APPLICATION OF ANTI-HBcIgA IN SERUM BY INDIRECT-ELISA

Objective:To modify a method of detecting anti-HBcIgA and to study the relationship between anti-HBcIgA and damage level of liver. Methods:The serum samples from 280 cases were detected by newly established indirect-ELISA and pretreating patients' serum and using sheep anti-human IgA-HRP (SAH-IgA-HRP) as indicator system. Results: Compared with the method of ACST-ELISA, the positive rate and S/N ratio detected by two methods were not significantly different(P>0. 05). The pos-itive rate of anti-HBcIgA and S/N ratio in serous hepatitis B,chronic active hepatitis B associated with cirrhosis,acute hepatitis B and chronic persistant hepatitis B were significantly higher than those in car-riers (P<0. 01),and also significantly higher in chronic active hepatitis B than those in chronic persis-tant hepatitis B (P<0. 01). Conclusion: The indirect-ELISA for detecting anti-HBcIgA is a sensitive,simple and rapid method instead of ACST-ELISA. Moreover,the positive rate and S/N ratio in anti-HB-cIgA are much higher in severe hepatitis than those in mild ones.,