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Green Rice Leafhopper Resistance Gene Transferring Through Backcrossing and CAPS Marker Assisted Selection
Authors:WANG Chun-ming  Hideshi Yasui  Atsushi Yoshimura  SU Chang-chao  ZHAI Hu-qu  WAN Jian-min
Abstract:Grh2, a green rice leafhopper resistant gene from an indica cultivar DV85, was located onchromosome 11, and two RFLP markers C189 and G1465 were found to be linked to this gene. In order totransfer Grh2 into Taichung65, a japonica cultivar with elite characters, backcross method with Taichung65as the recurrent parent was used and the two RFLP markers were converted into CAPS markers for marker as-sisted selection (MAS). In the BC6F3 population, both phenotypic evaluation and MAS were conducted toscreen the resistant plants with Taichung65 background. The linkage distance between CAPS markers andGrh2 was calculated and the efficiency of MAS was analyzed.
Keywords:Oryza sativa  Nephotettix virescens Uhler  Insect resistance  CAPS  Marker assisted selec-tion
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