| Identification of QTLs Related to Bolting in Brassica rapa ssp. pekinensis （syn. Brassica campestris ssp. pekinensis） |
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| 作者姓名： | ZHANG Xiao-wei WU Jian ZHAO Jian-jun SONG Xiao-fei LI Ying ZHANG Yan-guo XU Dong-hui SUN Ri-fei YUAN Yu-xiang XIE Cong-hua WANG Xiao-wu |
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| 作者单位： | [1]College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, P.R.China [2]Institute of Vegetables and Flowers. Chinese Academy of Agriculture Sciences, Beijing 100081, P.R.China [3]Institute of Biotechnology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China [4]Life Science College, Hebei Agricultural University, Baoding 071101, P.R.China |
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| 基金项目： | Acknowledgements The study was funded by the program of Strategic Scientific Alliances Between China and the Netherlands （973 International Cooperation Project, 2004CB720405）; The 0pening Lab of Vegetable Genetics and Physiology of Ministry of Agriculture; and the Sino-Dutch Joint Lab of Horticultural Genomics Technology, P.R.China. |
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| 摘 要： | A genetic linkage map of Brassica rapa ssp. pekinensis was constructed with 186 AFLP （amplified fragment length polymorphism） markers by using a doubled-haploid （DH） population with 183 individuals. The individuals were derived from F1 which was developed by crossing a bolting resistant DH line Y-177-12 and an easy bolting DH line Y195-93a. AFLPs were generated by the use of restriction enzymes EcoR Ⅰ and Mse Ⅰ . The segregation of each marker and linkage was analyzed by using JoinMap version 3.0. Mapped markers were aligned in ten linkage groups which covered 887.8 cM with an average marker interval of 4.47 cM. Markers showing skewed segregation ratio were clustered in six LGs. Quantitative trait loci （QTL） were mapped for bolting resistance by using MAPQTL 4.0 package. Four QTLs explaining from 7.0 to 9.4% of the total variation were detected, all of them increase bolting resistance. These mapped QTLs could be used to develop a marker assisted selection programme for bolting resistance breeding.
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| 关 键 词： | QTL 连锁图 甘蓝 蔬菜 多态性 |
| 收稿时间： | 2005-07-28 |
| 修稿时间： | 2006-02-25 |
Identification of QTLs Related to Bolting in Brassica rapa ssp. pekinensis (syn. Brassica campestris ssp. pekinensis) |
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| ZHANG Xiao-wei WU Jian ZHAO Jian-jun SONG Xiao-fei LI Ying ZHANG Yan-guo XU Dong-hui SUN Ri-fei YUAN Yu-xiang XIE Cong-hua WANG Xiao-wu.Identification of QTLs Related to Bolting in Brassica rapa ssp. pekinensis (syn. Brassica campestris ssp. pekinensis)[J].Agricultural Sciences in China,2006,5(4):265-271. |
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| Authors: | Xiao-wei ZHANG Jian WU Jian-jun ZHAO Xiao-fei SONG Ying LI Yan-guo ZHANG Dong-hui XU Ri-fei SUN Yu-xiang YUAN Cong-hua XIE Xiao-wu WANG |
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| Institution: | aCollege of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, P.R. China;bInstitute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R. China;cInstitute of Biotechnology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China;dLife Science College, Hebei Agricultural University, Baoding 071101, P.R. China |
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| Abstract: | A genetic linkage map of Brassica rapa ssp. pekinensis was constructed with 186 AFLP (amplified fragment length polymorphism) markers by using a doubled-haploid (DH) population with 183 individuals. The individuals were derived from F1 which was developed by crossing a bolting resistant DH line Y-177-12 and an easy bolting DH line Y195–93a. AFLPs were generated by the use of restriction enzymes EcoR I and Mse I. The segregation of each marker and linkage was analyzed by using JoinMap version 3.0. Mapped markers were aligned in ten linkage groups which covered 887.8 cM with an average marker interval of 4.47 cM. Markers showing skewed segregation ratio were clustered in six LGs. Quantitative trait loci (QTL) were mapped for bolting resistance by using MAPQTL 4.0 package. Four QTLs explaining from 7.0 to 9.4% of the total variation were detected, all of them increase bolting resistance. These mapped QTLs could be used to develop a marker assisted selection programme for bolting resistance breeding. |
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| Keywords: | bolting QTL linkage map Chinese cabbage |
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