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中国农业科学
作者单位:Lu Xinggui,Yuan Qianhua(Hubei Academy of Agricultural Sciences,Wuhan 430063);Yao Kemin,Mou Tongmin,Zong Xuemei(Nanjing Institute of Meteorology);Cao Bing(Hainan Academy of Agricultural Sciences);Huang Zonghong(Guizhou Academy of Agricultural Sciences)  
摘    要:In this experiment, the bovine follicular oocytes were aspirated from the ovaries of Chinese Holsteins with laparoscope made in China. The results were as following: for identifying the suitable negative aspiration pressure, six different pressures (50, 100, 150, 200, 250 and 300mmHg)were tested. The aspiration pressure of 100mmHg was the best. Its oocyte recovery rate was 37. 2%, and G I , G Ⅱ oocyte rate was 89. 5%. The heifers were picked up by laparoscope once or twice a week. Each heifer was collected with 2. 4 oocytes once a week or 4. 4 oocytes twice a week.Its oocyte recovery rate was 48. 0% and the G Ⅰ ,G Ⅱ oocyte rate was 93. 5%. In addition, 1.9 oocytes were collected from each cow once a week or 5.4 oocytes from each cow twice a week. Its oocyte recovery rate was 51.7% and the G Ⅰ , G Ⅱ oocyte rate was 85. 1%. It showed that it was possible to pick up bovine oocyte twice a week. Two cows were picked up twice a week for several weeks(53 times). 268 follicles were aspirated(5.1 follicles per cow per time), and 141 oocytes were recovered(2.7 oocytes per cow per time). The oocyte recovery rate was 52.5%, and the G Ⅰ , G Ⅱ oocyte rate was 85. 1%. It was advisable to pick up oocytes twice a week continuously. Some cows in estrous cycles were superovulated with PMSG(500IU). Each of them could be recovered 2.3 follicles and 1.1 oocytes, the others were superovulated with FSH(0. 7mg) , each of them could be aspirated with 4.4 follicles and 2.3 oocytes. It was obvious that the effect of OPU(oocyte pick up) by superovulation with FSH was much better than that with PMSG. The best time for OPU with laparoscope was at the beginning of cow's estrous cycles. At the first day of their estrus, each of them could be averagely aspirated with 8 follicles and 5.7 oocytes.


Studies on Molecular Variant Mechanism of Infectious Bronchitis Viruses of Beijing Area
Authors:Li Hua  Yang Hanchun
Abstract:Based on 13 genetic male sterile lines of paddy rice newly-developed in academies of agricultural sciences of China, study was undertaken of the determination of ecological conditions for their fertility conversion in terms of the leveling of daylength and temperature and a fertility quantifying model in the context of observed bagged seedlings (in percentage) through self mating by pollen inside, daylength and temperature from the experiments with sowing by stage in three different latitude zones, i. e., at Sanya of Hainan Province, Guiyang of Guizhou and Wuhan of Hubei. Results show that five sterile lines are temperature-sensitive with the fertility conversion temperature below 24 ℃, two of the daylength-sensitive sterile lines have conversion daylength of > 12. 4 h, suggesting their almost full sterility, and one of the photo- and temperature-sensitive lines needs shorter daylength either at high or at low temperature for fertility conversion. These eight sterile lines are of productive significance.
Keywords:Sterile line of rice  Fertility conversion index  Fertility quantifying model  Leveling of temperature and daylength
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