| Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut（Arachis hypogaea L.） Seeds |
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| 作者姓名： | PAN Li-juan YANG Qing-li CHI Xiao-yuan CHEN Ming-na YANG Zhen CHEN Na WANG Tong WANG Mian HE Ya-nan YU Shan-lin |
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| 作者单位： | Shandong Peanut Research Institute, Qingdao 266100, P.R. China |
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| 基金项目： | supported by the China Agriculture Research System （CARS-14）; the National Natural Science Foundation of China （31000728,31100205）; the Natural Science Fundation of Shangdong Province,China（ZR2009DQ004,ZR2011CQ036）; the Promotive Research Fund for Young and Middle-Aged Scientisits of Shandong Province,China （BS2010NY023）; the Qingdao Municipal Science and Technology Plan Project,China （11-2-4-9-（3）-jch,11-2-3-26-nsh）Acknowledgements This study was supported by the China Agriculture Research System （CARS-14）, the National Natural Science Foundation of China （31000728, 31100205）, the Natural Science Fundation of Shangdong Province, China （ZR2009DQ004, ZR2011 CQ036）, the Promotive Research Fund for Young and Middle-Aged Scientisits of Shandong Province, China （BS2010NY023）, and the Qingdao Municipal Science and Technology Plan Project, China （11-2-4-9- （3）-jch, 11-2-3-26-nsh）. |
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| 摘 要： | Phosphoenolpyruvate carboxylase(PEPC;EC 4.1.1.31) catalyses phosphoenolpyruvate(PEP) to yield oxaloacetate,which is involved in protein biosynthesis.Pyruvate kinase(PK;EC 2.7.1.40) catalyzes PEP to yield pyruvate,which is involved in fatty acid synthesis.In this study,five PEPC genes(AhPEPC1,AhPEPC2,AhPEPC3,AhPEPC4,and AhPEPC5) from peanut have been cloned.Using a quantitative real-time RT-PCR approach,the expression pattern of each gene was monitored during the seed development of four peanut varieties(E11,Hebeigaoyou,Naihan 1,and Huayu 26).It was found that these five genes shared similar expression behaviors over the developmental stages of E11 with high expression levels at 30 and 40 d after pegging(DAP);whereas these five genes showed irregular expression patterns during the seed development of Hebeigaoyou.In Naihan 1 and Huayu 26,the expression levels of the five genes remained relatively high in the first stage.The PEPC activity was monitored during the seed development of four peanut varieties and seed oil content was also characterized during whole period of seed development.The PEPC activity followed the oil accumulation pattern during the early stages of development but they showed a significantly negative correlation thereafter.These results suggested that PEPC may play an important role in lipid accumulation during the seed development of four peanut varieties tested.
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| 关 键 词： | peanut(Arachis hypogaea L.) phosphoenolpyruvate carboxylase PEPC activity lipid accumulation |
Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut（Arachis hypogaea L.） Seeds |
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| PAN,Li-juan YANG,Qing-li CHI,Xiao-yuan CHEN,Ming-na YANG,Zhen CHEN,Na WANG,Tong WANG,Mian HE,Ya-nan YU,Shan-lin.Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut（Arachis hypogaea L.） Seeds[J].Agricultural Sciences in China,2013(1):36-44. |
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| Authors: | Agricultural Sciences in China |
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| Institution: | PAN Li-juan,YANG Qing-li,CHI Xiao-yuan,CHEN Ming-na,YANG Zhen,CHEN Na,WANG Tong,WANG Mian,HE Ya-nan and YU Shan-lin Shandong Peanut Research Institute,Qingdao 266100,P.R.China |
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| Abstract: | |
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| Keywords: | peanut（Arachis hypogaea L ） phosphoenolpyruvate carboxylase PEPC activity lipid accumulation |
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