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| Inheritance Analysis and Identification of SSR Markers Linked to Late Blight Resistant Gene in Tomato | |
| 作者姓名： | ZHU Hai-shan WU Tao ZHANG Zhen-xian |
| 作者单位： | [1]Department of Vegetable, College of Agronomy and Bio-Technology, China Agricultural University, Beijing 100094, P.R.China [2]Department of Horticulture, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, P.R.China |
| 摘 要： | Late blight caused by Phytophthora infestans is the most serious disease of tomato production in China. Studies on the genetics of resistance and identification of molecular markers are very useful for breeding late blight resistant varieties. The objective of this paper was to study the inheritance of late blight resistance and identify simple sequence repeat （SSR） markers associated with resistance allele in tomato （Lycopersicon esculentum Mill）. The results came from an F2 progeny of 241 plants derived from a cross between 5~ inbred line that is susceptible to late blight and a resistant accession CLN2037E. The late blight responses of F2 plants were tested by artificially inoculation of detached-leaflets in plate and natural infection assayed under greenhouse conditions. Both methods showed that the resistance is dominant and inherited as monogenic trait. Genetic mapping and linkage analysis showed that the late blight resistance gene Ph-ROL was located on chromosome 9 with a genetic distance of 5.7 cM to the SSR marker TOM236. |
| 关 键 词： | 遗传特性 SSR 分子辅助标记 马铃薯 抗性基因 |
| 收稿时间： | 2006-02-17 |
| 修稿时间： | 2006-04-27 |
Inheritance Analysis and Identification of SSR Markers Linked to Late Blight Resistant Gene in Tomato |
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| ZHU Hai-shan WU Tao ZHANG Zhen-xian.Inheritance Analysis and Identification of SSR Markers Linked to Late Blight Resistant Gene in Tomato[J].Agricultural Sciences in China,2006,5(7):517-521. | |
| Authors: | ZHU Hai-shan WU Tao ZHANG Zhen-xian |
| Institution: | 1. Department of Vegetable, College of Agronomy and Bio-Technology, China Agricultural University, Beijing 100094, P.R. China;Department of Horticulture, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, P.R. China 2. Department of Horticulture, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, P.R. China 3. Department of Vegetable, College of Agronomy and Bio-Technology, China Agricultural University, Beijing 100094, P.R. China |
| Abstract: | Late blight caused by Phytophthora infestans is the most serious disease of tomato production in China. Studies on the genetics of resistance and identification of molecular markers are very useful for breeding late blight resistant varieties. The objective of this paper was to study the inheritance of late blight resistance and identify simple sequence repeat (SSR) markers associated with resistance allele in tomato (Lycopersicon esculentum Mill). The results came from an F2 progeny of 241 plants derived from a cross between 5# inbred line that is susceptible to late blight and a resistant accession CLN2037E. The late blight responses of F2 plants were tested by artificially inoculation of detached-leaflets in plate and natural infection assayed under greenhouse conditions. Both methods showed that the resistance is dominant and inherited as monogenic trait. Genetic mapping and linkage analysis showed that the late blight resistance gene Ph-ROL was located on chromosome 9 with a genetic distance of 5.7 cM to the SSR marker TOM236. |
| Keywords: | disease resistance late blight Phytophthora infestans SSR tomato |
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