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| AFLP and SCAR Markers Associated with Peel Color in Eggplant （Solanum melongena） | |
| 作者姓名： | LIAO Yi SUN Bao-juan SUN Guang-wen LIU Hou-cheng LI Zhi-liang LI Zhen-xing WANG Guo-ping CHEN Ri-yuan |
| 作者单位： | LIAO Yi(College of Horticulture,South China Agricultural University,Guangzhou 510642,P.R.China;Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,P.R.China);SUN Bao-juan,LI Zhi-liang,LI Zhen-xing(Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,P.R.China);SUN Guang-wen,LIU Hou-cheng,WANG Guo-ping,CHEN Ri-yuan(College of Horticulture,South China Agricultural University,Guangzhou 510642,P.R.China) |
| 基金项目： | The work was jointly supported by the Research Fund of Higher Education of Guangdong Province, China （cgzhzd0406） and Agriculture Research Foundation of Guangdong Province, China （2007A020200005-2）. |
| 摘 要： | Peel color is an important breeding objective for eggplant. Dark purple and purplish red are the most common colors in commercial eggplant cultivars. A co-dominant amplified fragment length polymorphism （AFLP） marker which was associated with the peel color （each in coupling phase to dark purple and purplish red） was found in studying the genetic diversity in 58 eggplant accessions （contained cultivars and wild relatives）. The maker bands were sequenced and converted to SCAR marker, this polymorphism in sequence was from an inserted/deleted （indels） mutation. And DNA from 136 eggplant materials （inbred lines, F1, and wild relatives） were amplified with the designed SCAR primers as template, high correlation between the SCAR marker and peel color （dark purple and purplish red） was found. Then, bulked line analysis （BLA） combined with AFLP was further used to identify polymorphic fragments, and another six AFLP markers were tested and verified to be associated with peel color, which demonstrated that BLA was an useful method for identifying molecular markers of interested traits. In conclusion, these markers will facilitate the MAS （maker-assisted selection） of eggplant breeding for peel color. |
| 关 键 词： | SCAR标记 AFLP标记 果皮颜色 茄子 扩增片段长度多态性 标记辅助选择 育种目标 遗传多样性 |
AFLP and SCAR Markers Associated with Peel Color in Eggplant(Solanum melongena) |
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| LIAO Yi,SUN Bao-juan,SUN Guang-wen,LIU Hou-cheng,LI Zhi-liang,LI Zhen-xing,WANG Guo-ping,CHEN Ri-yuan.AFLP and SCAR Markers Associated with Peel Color in Eggplant(Solanum melongena)[J].Agricultural Sciences in China,2009,8(12):1466-1474. | |
| Authors: | LIAO Yi SUN Bao-juan SUN Guang-wen LIU Hou-cheng LI Zhi-liang LI Zhen-xing WANG Guo-ping CHEN Ri-yuan |
| Institution: | 1. College of Horticulture,South China Agricultural University,Guangzhou 510642,P.R.China;Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,P.R.China 2. Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,P.R.China 3. College of Horticulture,South China Agricultural University,Guangzhou 510642,P.R.China |
| Abstract: | Peel color is an important breeding objective for eggplant. Dark purple and purplish red are the most common colors in commercial eggplant cultivars. A co-dominant amplified fragment length polymorphism (AFLP) marker which was associated with the peel color (each in coupling phase to dark purple and purplish red) was found in studying the genetic diversity in 58 eggplant accessions (contained cultivars and wild relatives). The maker bands were sequenced and converted to SCAR marker, this polymorphism in sequence was from an inserted/deleted (indels) mutation. And DNA from 136 eggplant materials (inbred lines, F1, and wild relatives) were amplified with the designed SCAR primers as template, high correlation between the SCAR marker and peel color (dark purple and purplish red) was found. Then, bulked line analysis (BLA) combined with AFLP was further used to identify polymorphic fragments, and another six AFLP markers were tested and verified to be associated with peel color, which demonstrated that BLA was an useful method for identifying molecular markers of interested traits. In conclusion, these markers will facilitate the MAS (maker-assisted selection) of eggplant breeding for peel color. |
| Keywords: | eggplant peel color bulked line analysis AFLP marker SCAR marker |
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