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Sequence Variations in the Bovine IGF-Ⅰ and IGFBP3 Genes and Their Association with Growth and Development Traits in Chinese Beef Cattle
作者单位:[1]Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China [2]Life Science Department, Luoyang Normal College, Luoyang 471022, P.R.China [3]College of Animal Science, Northwest A&F University, Yangling 712100, P.R. China
摘    要:The objective of this study was to determine the genotype effects of the bovine insulin-like growth factor Ⅰ (IGF-Ⅰ) and its binding protein 3 (IGFBP3) genes on growth and development traits in beef cows, including 130 Chinese Simmental, 42 Nanyang, and 47 Luxi Yellow cattle. Sequence variations in the bovine IGF-Ⅰ and IGFBP3 genes were investigated by single strand conformation polymorphism (SSCP). SSCPs were detected in 6 fragments, which is the 5'-flanking region, the 2nd exon, the 5th exon, and the 5th intron of the IGF-1 gene, and the 2nd exon, the 3rd exon of the 1GFBP3 gene. Two polymorphisms, an A-to-G transition in the 2rid exon of the IGF-Ⅰ gene and a T-to-C transition in the 2rid exon of IGFBP3 gene were detected in 3 breeds. The allele frequencies of 2 polymorphisms were 0.0411 (A), 0.9589 (B), and 0.7237 (A), 0.2763 (B), respectively. These 2 loci were analyzed to associate with body weight, height at withers, body length, heart girth, rump width, and beef production index (BPI) at 0, 6, 12, 24, and 36-month old. The IGFBP3 locus was shown to be associated with rump width, heart girth at 24-month and 36-month. Animals with BB genotype had higher rump width (24.86 ± 0.47) cm at 24-month and (27.50 ± 0.63) em at 36-month. The heart girth was highest for the individuals with BB genotype (171.33 ± 1.84) cm and higher than those with AB genotype (166.68 ± 1.13) cm (P〈 0.05) at 36-month.

关 键 词:胰岛素样生长因子  IGFBP3  中国西门塔尔牛  生长发育性状  基因型  序列变异  肉牛  单链构象多态性
收稿时间:14 October 2008

Sequence Variations in the Bovine IGF-Ⅰ and IGFBP3 Genes and Their Association with Growth and Development Traits in Chinese Beef Cattle
Authors:GAO Xue SHI Ming-yan XU Xiu-rong LI Jun-ya REN Hong-yan XU Shang-zhong
Institution:[1]Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China [2]Life Science Department, Luoyang Normal College, Luoyang 471022, P.R.China [3]College of Animal Science, Northwest A&F University, Yangling 712100, P.R. China
Abstract:The objective of this study was to determine the genotype effects of the bovine insulin-like growth factor I (IGF-I) and its binding protein 3 (IGFBP3) genes on growth and development traits in beef cows, including 130 Chinese Simmental, 42 Nanyang, and 47 Luxi Yellow cattle. Sequence variations in the bovine IGF-I and IGFBP3 genes were investigated by single strand conformation polymorphism (SSCP). SSCPs were detected in 6 fragments, which is the 5′-flanking region, the 2nd exon, the 5th exon, and the 5th intron of the IGF-I gene, and the 2nd exon, the 3rd exon of the IGFBP3 gene. Two polymorphisms, an A-to-G transition in the 2nd exon of the IGF-I gene and a T-to-C transition in the 2nd exon of IGFBP3 gene were detected in 3 breeds. The allele frequencies of 2 polymorphisms were 0.0411 (A), 0.9589 (B), and 0.7237 (A), 0.2763 (B), respectively. These 2 loci were analyzed to associate with body weight, height at withers, body length, heart girth, rump width, and beef production index (BPI) at 0, 6, 12, 24, and 36-month old. The IGFBP3 locus was shown to be associated with rump width, heart girth at 24-month and 36-month. Animals with BB genotype had higher rump width (24.86 ± 0.47) cm at 24-month and (27.50 ± 0.63) cm at 36-month. The heart girth was highest for the individuals with BB genotype (171.33 ± 1.84) cm and higher than those with AB genotype (166.68 ± 1.13) cm (P < 0.05) at 36-month.
Keywords:beef cattle  IGF-1 gene  1GFBP3 gene  growth and development traits  PCR-SSCP
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