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Construction of Genetic Linkage Map Based on SSR Markers in Peanut (Arachis hypogaea L.)
作者姓名:HONG  Yan-bin  LIANG  Xuan-qiang  CHEN  Xiao-ping  LIU  Hai-yan  ZHOU  Gui-yuan  LI  Shao-xiong  WEN  Shi-jie
作者单位:Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China
摘    要:Molecular genetic maps of crop species can be used in a variety of ways in breeding and genomic research such as identification and mapping of genes and quantitative trait loci (QTLs) for morphological, physiological and economic traits of crop species. However, a comprehensive genetic linkage map for cultivated peanut has not yet been developed due to the extremely low frequency of DNA polymorphism in cultivated peanut. In this study, 142 recombinant inbred lines (RILs) derived from a cross between Yueyou 13 and Zhenzhuhei were used as mapping population in peanut (Arachis hypogaea L.). A total 652 pairs of genomic-SSR primer and 392 pairs of EST-SSR primer were used to detect the polymorphisms between the two parents. 141 SSR primer pairs, 127 genomic-SSR and 14 EST-SSR ones, which can be used to detect polymorphisms between the two parents, were selected to analyze the RILs population. Thus, a linkage genetic map which consists of 131 SSR loci in 20 linkage groups, with a coverage of 679 cM and an average of 6.12 cM of inter-maker distance was constructed. The putative functions of 12 EST-SSR markers located on the map were analyzed. Eleven showed homology to gene sequences deposited in GenBank. This is the first report of construction of a comprehensive genetic map with SSR markers in peanut (Arachis hypogaea L.). The map presented here will provide a genetic framework for mapping the qualitative and quantitative trait in peanut.

关 键 词:花生  遗传学  遗传连锁图  SSR
收稿时间:22 April 2008

Construction of Genetic Linkage Map Based on SSR Markers in Peanut (Arachis hypogaea L.)
HONG Yan-bin LIANG Xuan-qiang CHEN Xiao-ping LIU Hai-yan ZHOU Gui-yuan LI Shao-xiong WEN Shi-jie.Construction of Genetic Linkage Map Based on SSR Markers in Peanut (Arachis hypogaea L.)[J].Agricultural Sciences in China,2008,7(8):915-921.
Authors:Yan-bin HONG  Xuan-qiang LIANG  Xiao-ping CHEN  Hai-yan LIU  Gui-yuan ZHOU  Shao-xiong LI  Shi-jie WEN
Institution:aCrops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R. China
Abstract:Molecular genetic maps of crop species can be used in a variety of ways in breeding and genomic research such as identification and mapping of genes and quantitative trait loci (QTLs) for morphological, physiological and economic traits of crop species. However, a comprehensive genetic linkage map for cultivated peanut has not yet been developed due to the extremely low frequency of DNA polymorphism in cultivated peanut. In this study, 142 recombinant inbred lines (RILs) derived from a cross between Yueyou 13 and Zhenzhuhei were used as mapping population in peanut (Arachis hypogaea L.). A total 652 pairs of genomic-SSR primer and 392 pairs of EST-SSR primer were used to detect the polymorphisms between the two parents. 141 SSR primer pairs, 127 genomic-SSR and 14 EST-SSR ones, which can be used to detect polymorphisms between the two parents, were selected to analyze the RILs population. Thus, a linkage genetic map which consists of 131 SSR loci in 20 linkage groups, with a coverage of 679 cM and an average of 6.12 cM of inter-maker distance was constructed. The putative functions of 12 EST-SSR markers located on the map were analyzed. Eleven showed homology to gene sequences deposited in GenBank. This is the first report of construction of a comprehensive genetic map with SSR markers in peanut (Arachis hypogaea L.). The map presented here will provide a genetic framework for mapping the qualitative and quantitative trait in peanut.
Keywords:peanut (Arachis hypogaea L  )  SSR  genetic linkage map
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