Analysis of Developmental Proteome at Egg Stage of Drone Honeybees （A. m. ligustica）

An investigation on the proteome of drone egg development of native Italian bee （Apis mellifera ligustica Spinola,1806） was carried out in order to prove up the characteristics in protein expression and regulation at egg stage and open out the molecular mechanism of the development. The experiment was carried out by two-dimensional gel electrophoresis. The results showed that there were 200, 242 and 233 proteins in a wide rang of molecular weight （12.42-169.60 kDa） and in a relatively narrow scope of pI （4.50-9.00） detected on day 1, day 2 and day 3, respectively, during the developmental process of the drone egg. Meanwhile, 164 protein spots were resolved at all the images （i.e., the protein was consistently expressed） along with the egg development, among which 7 were significantly up-expressed （P 〈 0.05） and 4 were significantly down-expressed （P 〈 0.05） while 79 had no significant differences （P 〉 0.05）. In addition, the specific proteins expressing proteins on day 1, day 2 and day 3 were 11, 18 and 18, respectively. Besides, 17 proteins expressed both on day 1 and day 2 but silenced on day 3, and 43 proteins expressed both on day 2 and day 3 but silenced on day 1, while only 8 proteins expressed both on day 1 and day 3 but silenced on day 2. The results indicate that 2-d-old eggs are at the most active expressional stage in the development of drone egg. The protein expressing at all images suggests that it should be indispensable for drone egg development, but their expression pattern is different. The proteins expressing at a specific age of egg suggest that specific proteins are needed in different developmental stages to regulate. And there are more house-keeping proteins in the developmental process of the drone egg than that of worker egg, and it will provide more targets for gene improvement.

Analysis of Developmental Proteome at Egg Stage of Drone Honeybees(A. m. ligustica)

An investigation on the proteome of drone egg development of native Italian bee (Apis mellifera ligustica Spinola,1806) was carried out in order to prove up the characteristics in protein expression and regulation at egg stage and open out the molecular mechanism of the development. The experiment was carried out by two-dimensional gel electrophoresis. The results showed that there were 200,242 and 233 proteins in a wide rang of molecular weight (12.42-169.60 kDa) and in a relatively narrow scope of pI (4.50-9.00) detected on day 1,day 2 and day 3,respectively,during the developmental process of the drone egg. Meanwhile,164 protein spots were resolved at all the images (I.e.,the protein was consistently expressed) along with the egg development,among which 7 were significantly up-expressed (P< 0.05) and 4 were significantly down-expressed (P<0.05) while 79 had no significant differences (P>0.05). In addition,the specific proteins expressing proteins on day 1,day 2 and day 3 were 11,18 and 18,respectively. Besides,17 proteins expressed both on day 1 and day 2 but silenced on day 3,and 43 proteins expressed both on day 2 and day 3 but silenced on day 1,while only 8 proteins expressed both on day 1 and day 3 but silenced on day 2. The results indicate that 2-d-old eggs are at the most active expressional stage in the development of drone egg. The protein expressing at all images suggests that it should be indispensable for drone egg development,but their expression pattern is different. The proteins expressing at a specific age of egg suggest that specific proteins are needed in different developmental stages to regulate. And there are more house-keeping proteins in the developmental process of the drone egg than that of worker egg,and it will provide more targets for gene improvement.