| Sequencing, Polymorphism and Expression Profile Analysis of Porcine Hexokinase Ⅱ （HK2） Gene |
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| 作者姓名： | WANG Jun DENG Chang-yan XIONG Yuan-zhu ZUO Bo CHENG Huan-chen LI Feng-e LEI Ming-gang ZHENG Rong JIANG Si-wen |
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| 作者单位： | Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture/College of Animal Sciences, Huazhong Agricultural University,Wuhan 430070, P.R. China |
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| 基金项目： | Acknowledgements We would like to thank the staff at Jingpin Pig Station, Huazhong Agriculture University, and teachers and graduate students at Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture for managing and slaughtering the research flocks. This study was supported financially by the National 973 Project, China （G2000016105）, National High Technology Development Project （2003AA243030） and and the Natural Science Foundation of Hubei Province, China （2005ABA142）. |
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| 摘 要： | Hexokinase Ⅱ has been demonstrated to play the role of a key enzyme member in the glycolysis reaction. It catalyzes the conversion of glucose to glucose-6-phosphate, thus committing glucose to the glycolytic pathway. In this paper, the partial exons and introns 10, 11, 13 and 14 of the porcine HK2 gene were cloned and sequenced by comparative genomics. Comparative sequencing of three pig breeds revealed ten putative single-nucleotide polymorphisms （SNPs）, one of which in intron 10 with differing bases （G981A） is within the restriction site for enzyme Msp I. Distribution of Msp I -RFLP genotype and allele frequencies among different pig breeds were studied. By association analysis between Msp I PCR- RFLP polymorphism （AA, AB, BB genotypes of HK2 gene intron 10） and some meat quality and carcass traits in F2 group, which was constructed by our laboratory, a significant difference of pig average backfat at rump was found between AB and BB genotypes （P〈0 05） in F2 group. In addition, the pattern of expression of ilK2 in a variety of tissues in pig was also determined using semi-quantitative RT-PCR. The expression of HK2 mRNA was detected only in pig skeletal muscle.
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| 关 键 词： | 己糖激酶 猪 SNPs 骨骼肌 |
| 收稿时间： | 2005-06-29 |
| 修稿时间： | 2006-12-26 |
Sequencing, Polymorphism and Expression Profile Analysis of Porcine Hexokinase II (HK2) Gene |
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| WANG Jun DENG Chang-yan XIONG Yuan-zhu ZUO Bo CHENG Huan-chen LI Feng-e LEI Ming-gang ZHENG Rong JIANG Si-wen.Sequencing, Polymorphism and Expression Profile Analysis of Porcine Hexokinase II (HK2) Gene[J].Agricultural Sciences in China,2006,5(5):384-389. |
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| Authors: | Jun WANG Ph D candidate Chang-yan DENG Yuan-zhu XIONG Bo ZUO Huan-chen CHENG Feng-e LI Ming-gang LEI Rong ZHENG Si-wen JIANG |
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| Institution: | aKey Laboratory of Swine Genetics and Breeding, Ministry of Agriculture/College of Animal Sciences, Huazhong Agricultural University, Wuhan 430070, P.R.China |
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| Abstract: | Hexokinase II has been demonstrated to play the role of a key enzyme member in the glycolysis reaction. It catalyzes the conversion of glucose to glucose-6-phosphate, thus committing glucose to the glycolytic pathway. In this paper, the partial exons and introns 10, 11, 13 and 14 of the porcine HK2 gene were cloned and sequenced by comparative genomics. Comparative sequencing of three pig breeds revealed ten putative single-nucleotide polymorphisms (SNPs), one of which in intron 10 with differing bases (G981A) is within the restriction site for enzyme Msp I. Distribution of Msp I -RFLP genotype and allele frequencies among different pig breeds were studied. By association analysis between Msp I PCR-RFLP polymorphism (AA, AB, BB genotypes of HK2 gene intron 10) and some meat quality and carcass traits in F2 group, which was constructed by our laboratory, a significant difference of pig average backfat at rump was found between AB and BB genotypes (P<0 05) in F2 group. In addition, the pattern of expression of HK2 in a variety of tissues in pig was also determined using semi-quantitative RT-PCR. The expression of HK2 mRNA was detected only in pig skeletal muscle. |
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| Keywords: | hexokinase II pig SNPs expression profile skeletal muscle |
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