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| QTL Mapping of Isoflavone,Oil and Protein Contents in Soybean （Glycine max L. Merr.） | |
| 作者姓名： | LIANG Hui-zhen YU Yong-liang WANG Shu-feng LIAN Yun WANG Ting-feng WEI Yan-li GONG Peng-tao LIU Xue-yi FANG Xuan-jun ZHANG Meng-chen |
| 作者单位： | [1]Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/National Soybean Improvement Sub-Center of Zhengzhou Zhengzhou 450002, P.R.China [2]Hainan Institute of Tropieal Agriculture Resources, Sanya 572025, P.R. China [3]Economical Crops Institute, Shanxi Academy of Agricultural Sciences, Fenyang 032200, P.R.China [4]Institue of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050031, P.R. China |
| 基金项目： | China，the National 948 Project of China，the National 973 Program of China，the Henan Province key Scientific and Technological Project;China，the Transformational Fund of Agricultural Scientific and Technological Achievements in China，The National Key Technologies R&D Program of China |
| 摘 要： | Soybean （Glycine max L. Merr.） is the world＇s foremost source of edible plant oil and proteins, meantime, the biologically active secondary metabolites such as saponins and isoflavones are benefit to human health. The objective of this study was to identify quantitative trait loci （QTL） and epistatic interactions associated with isoflavone, protein, and oil contents in soybean seeds. An F13 recombinant inbred line （RIL） comprising 474 lines was derived from a cross between Jindou 23 and Huibuzhi cultivars. SSR technique was employed for mapping of the QTLs. The QTLs for isoflavone, protein, and oil contents were analyzed and 23 QTLs were detected based on the constructed linkage map. Six QTLs for isoflavone content were localized in linkage groups J, N, D2, and G, eleven QTLs for oil content were localized in the linkage groups A1, A2, B2, C2, and D2, and six QTLs for protein content were localized in linkage groups B2, C2, G, and H1. The correlative analysis demonstrated that the isoflavone content had significant correlation with protein content, while significantly negative correlations was existed between oil and protein content, and significantly positive correlations was existed between protein and oil content. All these findings have laid an important basis for the marker assisted breeding in soybean. The phenotypic correlations of quantitative traits may be resulted from the correlation of the QTL controlling those traits. |
| 关 键 词： | QTL定位 蛋白质含量 异黄酮含量 大豆属 数量性状位点 SSR标记技术 石油 次生代谢产物 |
QTL Mapping of Isoflavone,Oil and Protein Contents in Soybean (Glycine max L.Merr.) |
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| LIANG Hui-zhen,YU Yong-liang,WANG Shu-feng,LIAN Yun,WANG Ting-feng,WEI Yan-li,GONG Peng-tao,LIU Xue-yi,FANG Xuan-jun,ZHANG Meng-chen.QTL Mapping of Isoflavone,Oil and Protein Contents in Soybean (Glycine max L.Merr.)[J].Agricultural Sciences in China,2010,9(8):1108-1116. | |
| Authors: | LIANG Hui-zhen YU Yong-liang WANG Shu-feng LIAN Yun WANG Ting-feng WEI Yan-li GONG Peng-tao LIU Xue-yi FANG Xuan-jun ZHANG Meng-chen |
| Institution: | 1. Industrial Crops Research Institute,Henan Academy of Agricultural Sciences/National Soybean Improvement Sub-Center of Zhengzhou,Zhengzhou 450002,P.R.China 2. Hainan Institute of Tropical Agriculture Resources,Sanya 572025,P.R.China 3. Economical Crops Institute,Shanxi Academy of Agricultural Sciences,Fenyang 032200,P.R.China 4. Institue of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031,P.R.China |
| Abstract: | Soybean (Glycine max L.Merr.) is the world's foremost source of edible plant oil and proteins,meantime,the biologically active secondary metabolites such as saponins and isoflavones are benefit to human health.The objective of this study was to identify quantitative trait loci (QTL) and epistatic interactions associated with isoflavone,protein,and oil contents in soybean seeds.An F13 recombinant inbred line (RIL) comprising 474 lines was derived from a cross between Jindou 23 and Huibuzhi cultivars.SSR technique was employed for mapping of the QTLs.The QTLs for isoflavone,protein,and oil contents were analyzed and 23 QTLs were detected based on the constructed linkage map.Six QTLs for isoflavone content were localized in linkage groups J,N,D2,and G,eleven QTLs for oil content were localized in the linkage groups A1,A2,B2,C2,and D2,and six QTLs for protein content were localized in linkage groups B2,C2,G,and H1.The correlative analysis demonstrated that the isoflavone content had significant correlation with protein content,while significantly negative correlations was existed between oil and protein content,and significantly positive correlations was existed between protein and oil content.All these findings have laid an important basis for the marker assisted breeding in soybean.The phenotypic correlations of quantitative traits may be resulted from the correlation of the QTL controlling those traits. |
| Keywords: | soybean SSR QTL quality isoflavone |
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