Cytological observation of the microspore development of Chinese kale and false pakchoi |
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Authors: | Zhijun Li Yanrong Zhang Chunyan Li Weiping Long Wengjia Lu Fuguang Han |
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Institution: | (1) Seed and Seedling Centre, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China |
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Abstract: | The development process and morphology of the microspores in Chinese kale and false pakchoi were observed by using the whole
stain-clearing technique. The results showed that the morphological characteristics of microspores were not only extremely
similar, but were also in their development processes. The microspores at tetrad stage showed an arrangement of tetrahedral
type, and after being released and passing through two mitoses, they developed gradually to form rotundly-shaped mature pollen
grains containing three nuclei, one bigger alimentation nucleus and two smaller similar-sized generative nuclei. Determination
of bud sizes at four typical microspore developmental stages revealed that the bud size had a stable heredity at each development
stage of the microspores. The ratio of the bud length at the late uninucleate stage to the largest bud length differed little
between Chinese kale and false pakchoi, ranging from 0.37 to 0.45 with an average of 0.41, though there was significant difference
among their cultivars. It was concluded that the length of buds at late uninucleate stage can be estimated for undetermined
cultivars of these two Brassica crops by multiplying the largest bud length with the following coefficient or regression equation: Y = 0.3898X + 0.1503, where X is the length of the largest bud. |
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Keywords: | Chinese kale false pakchoi microspore late uninucleate stage |
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