Cloning and structure analysis of an NBS-LRR disease-resistant gene from Setaria italica Beauv |
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| Authors: | Qiaoyun Weng Zhiyong Li Jihong Xing Zhiping Dong Jingao Dong |
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| Institution: | (1) Molecular Plant Pathology Lab, College of Life Science, Agricultural University of Hebei, Baoding, 071001, China;(2) Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, 050000, China |
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| Abstract: | Degenerate PCR primers targeting conserved motifs of most NBS-LRR disease-resistant genes in plants were tested in Setaria italica Beauv. cultivar Shilixiang, which is resistant to Uromyces setariae-italicae. A sequence with a length of 2673 bp has been obtained by using Genomic Walking technology. The nucleotide sequence contained
an open reading frame that encoded 891 amino acid residues with a calculated molecular mass of 101.44 kDa. It was named RUS1 (Resistance against Uromyces setariae-italicae, GenBank No. FJ467296). It contained an NB-ARC domain and three conserved motifs P-loop, kinase 2, and kinase 3, which had
the characteristics of NBS-LRR type resistant gene of plant. Phylogenetic analysis indicated that it was similar to RPM1 and might belong to LZ-NBS-LRR type disease resistance gene. Southern blotting result displayed that there were at least
three copies of RUS1 in the foxtail millet genome. |
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| Keywords: | Setaria italica RUS1 NB-ARC domain |
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