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转双价基因(Bt+CpTI)抗虫棉对棉蚜羧酸酯酶和乙酰胆碱酯酶比活力的影响(英文)
引用本文:薛丽,卢延,周正堂,高希武,宋敦伦.转双价基因(Bt+CpTI)抗虫棉对棉蚜羧酸酯酶和乙酰胆碱酯酶比活力的影响(英文)[J].Agricultural Science&Technology,2011(7).
作者姓名:薛丽  卢延  周正堂  高希武  宋敦伦
作者单位:中国农业大学农学与生物技术学院昆虫系;
基金项目:Supported by Major Program for New Transgenic Plant VarietiesBreeding (2008ZX08012-04)~~
摘    要:目的]研究了转双价基因棉SGK321对棉蚜Aphis gossypii Glover羧酸酯酶和乙酰胆碱酯酶比活力的影响,为转基因棉的生物安全性评价提供理论依据。方法]在SGK321及其亲本棉石远321上长期饲养棉蚜,研究2种棉花品种对棉蚜短期(第1、2、3代)和长期(第41、42、43代)的影响。结果]在SGK321上取食1代的棉蚜种群羧酸酯酶比活力显著高于在亲本棉上取食1、2、3代的棉蚜种群,转双价基因棉SGK321上饲养1、2、3代的棉蚜乙酰胆碱酯酶比活力均显著高于在石远321上饲养相同代数的棉蚜。但长期饲养的2种棉蚜之间羧酸酯酶比活力、乙酰胆碱酯酶比活力无显著差异。结论]长期取食转双价基因棉SGK321的棉蚜通过棉蚜解毒酶的调节作用对SGK321产生了适应性。

关 键 词:转双价基因棉  棉蚜  羧酸酯酶  乙酰胆碱酯酶

Effect of Transgenic Bt plus CpTI Cotton on Carboxylesterase and Acetylcholinesterase of Cotton Aphid Aphis gossypii
XUE Li,LU Yan,ZHOU Zheng-tang,GAO Xi-wu,SONG Dun-lun.Effect of Transgenic Bt plus CpTI Cotton on Carboxylesterase and Acetylcholinesterase of Cotton Aphid Aphis gossypii[J].农业科学与技术(英文版),2011(7).
Authors:XUE Li  LU Yan  ZHOU Zheng-tang  GAO Xi-wu  SONG Dun-lun
Institution:XUE Li,LU Yan,ZHOU Zheng-tang,GAO Xi-wu,SONG Dun-lun* Department of Entomology,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193
Abstract:Objective] The research aimed to assess the effect of transgenic Bt plus CpTI cotton variety SGK321 on carboxylesterase and acetylcholinesterase of cotton aphid Aphis gossypii and provide theoretical basis for studying the biosafety of transgenic cotton.Method] Cotton aphids were fed with SGK321 and Shiyuan321(normal parental varieties) for over 40 generations.Enzyme activities were compared between cotton aphids feeding on SGK321 for 1,2,3,41,42 and 43 generations with those on Shiyuan321.Result] The ca...
Keywords:Transgenic Bt plus CpTI cotton  Aphis gossypii Glover  Carboxylesterase  Acetylcholinesterase  
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