| 半夏疏松愈伤组织诱导条件的优化研究(英文) |
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| 引用本文： | 朱艳芳,毛春娜,张爱民,薛建平,李佳娣,宋运贤.半夏疏松愈伤组织诱导条件的优化研究(英文)[J].Agricultural Science&Technology,2011(8). |
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| 作者姓名： | 朱艳芳 毛春娜 张爱民 薛建平 李佳娣 宋运贤 |
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| 作者单位： | 淮北师范大学生命科学学院资源植物生物学安徽省重点实验室; |
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| 基金项目： | Supported by National Natural Science Fund Item(30973963); Anhui Natural Science Fund Item(090413252); Natural Science ResearchKey Project of Anhui Provincial University(KJ2009A160); Key Labora-tory Projectof Plant Resource and Biology(KLPRB200914)~~ |
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| 摘 要： | 目的]建立半夏细胞悬浮培养体系。方法]以半夏叶柄为外植体,采用正交试验设计研究了2,4-D、NAA、毒莠定和KT4种植物生长物质及其配比对疏松愈伤组织形成的影响。结果]2,4-D和毒莠定对半夏叶柄愈伤组织诱导效果最显著,KT和NAA次之。诱导半夏叶柄形成疏松愈伤组织的适宜培养基为MS+0.5mg/L2,4-D+1.0mg/LNAA+1.0mg/L毒莠定+1.5mg/LKT。结论]为通过半夏细胞悬浮提取有效成分和生产人工种子奠定了基础。
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| 关 键 词： | 半夏 叶柄 疏松愈伤组织 悬浮培养 |
Optimization of Inducing Conditions for Loose Callus of Pinellia ternata |
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| ZHU Yan-fang,MAO Chun-na,ZHANG Ai-min,XUE Jian-ping,LI Jia-di,SONG Yun-xian Anhui Key Laboratory of Plant Resources , Biology,School of Life Sciences,Huaibei Normal University,Huaibei.Optimization of Inducing Conditions for Loose Callus of Pinellia ternata[J].农业科学与技术(英文版),2011(8). |
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| Authors: | ZHU Yan-fang MAO Chun-na ZHANG Ai-min XUE Jian-ping LI Jia-di SONG Yun-xian Anhui Key Laboratory of Plant Resources Biology School of Life Sciences Huaibei Normal University Huaibei |
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| Institution: | ZHU Yan-fang,MAO Chun-na,ZHANG Ai-min,XUE Jian-ping,LI Jia-di,SONG Yun-xian Anhui Key Laboratory of Plant Resources and Biology,School of Life Sciences,Huaibei Normal University,Huaibei 235000 |
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| Abstract: | Objective] The research aimed to establish the cell suspension culture system of Pinellia ternate.Method] The petiole of Pinellia ternate was as the explant.The orthogonal test was used to study the influences of four plant growth substances(2,4-D,NAA,picloram and KT)and their mixture ratios on the formation of loose callus.Result] The induction effect of 2,4-D and picloram on the petiole callus of Pinellia ternate was the most significant.Then,the second ones were KT and NAA.The optimal medium which ind... |
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| Keywords: | Pinellia ternata Petiole Loose callus Suspension culture |
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