| 马铃薯三种病毒的多重PCR检测方法构建(英文) |
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| 引用本文： | 乔楠,曹佳,李霞.马铃薯三种病毒的多重PCR检测方法构建(英文)[J].Agricultural Science&Technology,2011(8). |
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| 作者姓名： | 乔楠 曹佳 李霞 |
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| 作者单位： | 榆林学院生命科学学院;北京真思维生物科技有限公司;陕西省榆林市农业科学研究所; |
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| 摘 要： | 目的]构建马铃薯A病毒(Potato virusA,PVA)、烟草花叶病毒(Tobacco mosaicvirus,TMV)、马铃薯Y病毒(Potato virusY,PVY)3种马铃薯主要易受感染的病毒的多重PRC(multiplex PCR)检测方法。方法]根据GenBank公布的PVA、TMV、PVY基因序列分别设计引物,建立3种马铃薯病毒的多重PCR方法;通过构建3种病毒PCR扩增目的基因的质粒用于制备标准品进行敏感性验证,利用PVX(Potato virus X)、PVM(Potato virus M)、PVS(Potato virus S)、PVV(Potato virus V),CMV(Cucumber mosaic virus)等其他马铃薯病毒进行特异性试验;并对11份疑似发生病毒病的马铃薯块茎采集样品进行了检测。结果]研究中构建的马铃薯3种病毒多重PCR检测方法,最低检测下限:PVA为100落copies/2μl,PVY为100copies/2μl,TMV为1000copies/2μl,与PVX、PVM、PVV、PVS、CMV等其他马铃薯病毒无交叉反应,具有良好的特异性;11份疑似发生病毒病的马铃薯块茎中7份样品检出3种病毒感染阳性。结论]研究成功构建了马铃薯PVA、TMV、PVY病毒的多重PCR方法,为马铃薯病毒检测技术应用奠定一定基础。
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| 关 键 词： | 马铃薯 PVA TMV PVY 多重PCR |
Establishment of Multiplex PCR for Three Virus of Potato |
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| QIAO Nan,CAO Jia,LI Xia .School of Life Science,Yulin University,Yulin.Establishment of Multiplex PCR for Three Virus of Potato[J].农业科学与技术(英文版),2011(8). |
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| Authors: | QIAO Nan CAO Jia LI Xia School of Life Science Yulin University Yulin |
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| Institution: | QIAO Nan1,CAO Jia2,LI Xia3 1.School of Life Science,Yulin University,Yulin 719000,2.Beijing Genes Way Co.,Ltd.,Beijing 100044,3.Yulin Institute of Agricultural Sciences |
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| Abstract: | Objective]The aim was to establish the multiplex PCR method for three virus of potato:PVA(potato virus A),TMV(Tobacco mosaic virus)and PVY(potato virus Y).Method]According to the PVA,TMV and PVY sequences available in GenBank,pairs of primer were designed for establishing a multiplex PCR method,and constructing recombinant plasmid of target genes by PCR amplified of three viruses as reference standard simple to be used in sensitivity test;PVX(Potato virus X),PVM(Potato virus M),PVS(Potato virus S),PVV(Pot... |
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| Keywords: | Potato Potato virus A Potato virus Y Tobacco mosaic virus Multiplex PCR |
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