| 水稻线粒体nad1基因超表达载体的构建和遗传转化(英文) |
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| 引用本文： | 李炜,谭艳平,王春台,刘学群.水稻线粒体nad1基因超表达载体的构建和遗传转化(英文)[J].Agricultural Science&Technology,2011(7). |
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| 作者姓名： | 李炜 谭艳平 王春台 刘学群 |
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| 作者单位： | 中南民族大学生命科学院/生物技术国家民委重点实验室; |
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| 基金项目： | Supported by the National Natural Science Foundation of China(30871318)~~ |
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| 摘 要： | 目的]克隆线粒体相关基因nad1,获得转nad1的转基因水稻植株。方法]采用TRIzol法提取水稻幼苗总RNA,以反转录的cDNA为模板,扩增得到nad1;将nad1接到线粒体信号肽Rf1b的5 (Rf1b5 ),装载到pCAMBIA1305.1双元表达载体,采用农杆菌介导的愈伤组织侵染法进行遗传转化。结果]克隆的目的基因nad1大小为978 bp,成功构建了携带线粒体信号肽的nad1植物表达载体,并获得了转nad1基因的阳性植株。结论]为探讨水稻中过表达nad1对水稻生长的影响奠定了基础。
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| 关 键 词： | 水稻 nad1 遗传转化 |
Construction and Genetic Transformation of Over-Expression Vector of nad1 Gene in Rice |
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| LI Wei,TAN Yan-ping,WANG Chun-tai,LIU Xue-qun Key Laboratory for Biotechnology of the State Ethnic Affairs Commission,College of Life Science,South-Central University for Nationalities,Wuhan.Construction and Genetic Transformation of Over-Expression Vector of nad1 Gene in Rice[J].农业科学与技术(英文版),2011(7). |
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| Authors: | LI Wei TAN Yan-ping WANG Chun-tai LIU Xue-qun Key Laboratory for Biotechnology of the State Ethnic Affairs Commission College of Life Science South-Central University for Nationalities Wuhan |
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| Institution: | LI Wei,TAN Yan-ping,WANG Chun-tai,LIU Xue-qun* Key Laboratory for Biotechnology of the State Ethnic Affairs Commission,College of Life Science,South-Central University for Nationalities,Wuhan 430074 |
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| Abstract: | |
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| Keywords: | Rice nad1 Genetic transformation |
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