| 新型甘蓝型油菜核不育材料绵7AB-4-2农艺性状鉴定及分子检测(英文) |
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| 引用本文： | 李浩杰,汤天泽,袁代斌,蒙大庆,蒲晓斌,张锦芳,蒋梁材.新型甘蓝型油菜核不育材料绵7AB-4-2农艺性状鉴定及分子检测(英文)[J].Agricultural Science&Technology,2009(1). |
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| 作者姓名： | 李浩杰 汤天泽 袁代斌 蒙大庆 蒲晓斌 张锦芳 蒋梁材 |
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| 作者单位： | 四川省农业科学院作物研究所;四川省绵阳农科所; |
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| 基金项目： | Supported by National High-tech R&D Program of China(2001AA241104, 2004AA241104);;Key Breeding Project of Sichuan Province (200107001-1-6-1, 2006YZGG-5);;Key Quality Project of Sichuan Province(2006YZGG-23)~~ |
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| 摘 要： | Objective] The study was to investigate the agronomic traits and breeding characteristics of genic male sterile material Mian 7AB-4-2 in Brassica napus. Method] The differences in agronomic traits and polymorphisms in SSR markers, between the genic male sterile material Mian 7AB-4-2 in Brassica napus and its sisterly line Mian 7AB-4-1 were investigated by hybridization and molecular identification; and the percentage of sterile individuals of Mian 7AB-4-2 and of the hybrids with its sisterly line Mian 7AB-4-1 from test cross and back cross were also studied. Result] Mian 7AB-4-2 was not significantly different in agronomic traits from its sisterly line Mian 7AB-4-1 at 0.05 probability level. The percentages of sterile individuals in the pollinated fertile Mian 7AB-4-2 plants were over 60%, and that in its sisterly line Mian 7AB-4-1 was about 25%. In test crosses with other nine sterile lines, Mian 7AB-4-1 kept the percentage of sterile individuals of sterile lines over 90%, and the percentage of sterile individuals from back cross over 80%. With regard to molecular markers, Mian 7AB-4-2 and its sisterly line Mian 7AB-4-1 were different in the band number from SSR primers a2 and E10. Conclusion] The results indicate that Mian 7AB-4-2 is helpful for rapeseed breeding, quickening the application of new materials in field breeding.
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| 关 键 词： | 多态性 不育株 兄妹交 不育性 隐性核不育系 农艺性状 材料与方法 可育株 分子标记 新材料 |
Molecular Markers and Agronomic Traits of a New Kind of Genic Male Sterile Material Mian 7AB-4-2 in Brassica napus L. |
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| LI Hao-jie,TANG Tian-ze,YUAN Dai-bin,MENG Da-qing,PU Xiao-bin,ZHANG Jin-fang,JIANG Liang-cai. Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu ,. Mianyang Institute of Agricultural Sciences,Mianyang.Molecular Markers and Agronomic Traits of a New Kind of Genic Male Sterile Material Mian 7AB-4-2 in Brassica napus L.[J].农业科学与技术(英文版),2009(1). |
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| Authors: | LI Hao-jie TANG Tian-ze YUAN Dai-bin MENG Da-qing PU Xiao-bin ZHANG Jin-fang JIANG Liang-cai Crop Research Institute Sichuan Academy of Agricultural Sciences Chengdu Mianyang Institute of Agricultural Sciences Mianyang |
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| Institution: | LI Hao-jie1,TANG Tian-ze2,YUAN Dai-bin2,MENG Da-qing2,PU Xiao-bin1,ZHANG Jin-fang1,JIANG Liang-cai11. Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066,2. Mianyang Institute of Agricultural Sciences,Mianyang,621023 |
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| Abstract: | Objective] The study was to investigate the agronomic traits and breeding characteristics of genic male sterile material Mian 7AB-4-2 in Brassica napus. Method] The differences in agronomic traits and polymorphisms in SSR markers, between the genic male sterile material Mian 7AB-4-2 in Brassica napus and its sisterly line Mian 7AB-4-1 were investigated by hybridization and molecular identification; and the percentage of sterile individuals of Mian 7AB-4-2 and of the hybrids with its sisterly line Mian 7AB... |
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| Keywords: | Brassica napus L Recessive genic male sterility Self-pollination of fertile individual Test cross Back cross SSR marker Polymorphism |
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