Polyploidy Induction of Pteroceltis tatarinowii Maxim.

Objective] This study was conducted to obtain tetraploid Pteroceltis tatarinowii Maxim. with excellent ornamental traits. Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with different concentrations of colchicine solution for different hours to figure out a proper method and obtain polyploids. Result] The most effective induction was obtained by treatment with 0.6%-0.8% colchicine for 72 h with 34.2% mutation rate. Flow cytometry and chromosome observation of the stem apex growing point of P. tatarinowii Maxim. proved that the tetraploid plants were successfully obtained with chromosome number 2n =4x =36.Conclusion] The result not only fills the blank of polyploid breeding of P. tatarinowii,but also provides an effective way to broaden the methods of cultivation of fastgrowing, high-quality, disease-resilience, new varieties of Pteroceltis.