| 罹病光肩星天牛幼虫分离菌株的16S rDNA系统发育分析(英文) |
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| 作者单位： | DENG Cai-ping1，LIU Hong-xia1，YAN Xi-zhong2，WU Xu-xia3，LUO You-qing11. Key Laboratory for Silviculture and Conservation of Education Ministry，Beijing Forestry University，Beijing 100083; 2. College of Agriculture，Shanxi Agricultural University，Taigu 030801; 3. Biological Application Engineering Department of Hohhot Vocation College，Hohhot 010051 |
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| 摘 要： | Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis(Motsch.) Method] The strain was identified by routine method and inoculated onto healthy Anoplophora glabripennis(Motsch.) for observing insecticidal effect,further 16S DNA was amplified by the specific primers for sequencing and homology analysis. Result] The mortality of second instar of Anoplophora glabripennis(Motsch.) reached 72.7% 8 d after 1010 cfu/ml BH-1 was inoculated. The homology of 16S DNA sequences between BH-1 and Serratia marcescens accessed in GenBank reached 99.5%. Combined with the results of routine identification,BH-1 was identified as S. marcescens. Conclusion] BH-1 could be used for biological control of Anoplophora glabripennis (Motsch.).
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Phylogenetic Analysis of the 16S rDNA of a Strain Isolated from Diseased Larva of Anoplophora glabripennis (Motsch.) |
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| Authors: | DENG Cai-ping LIU Hong-xia YAN Xi-zhong WU Xu-xia LUO You-qing Key Laboratory for Silviculture and Conservation of Education Ministry Beijing Forestry University Beijing College of Agriculture Shanxi Agricultural University Taigu Biological Application Engineering |
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| Institution: | DENG Cai-ping1,LIU Hong-xia1,YAN Xi-zhong2,WU Xu-xia3,LUO You-qing11. Key Laboratory for Silviculture and Conservation of Education Ministry,Beijing Forestry University,Beijing 100083, 2. College of Agriculture,Shanxi Agricultural University,Taigu 030801, 3. Biological Application Engineering Department of Hohhot Vocation College,Hohhot 010051 |
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| Abstract: | Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis(Motsch.) Method] The strain was identified by routine method and inoculated onto healthy Anoplophora glabripennis(Motsch.) for observing insecticidal effect,further 16S DNA was amplified by the specific primers for sequencing and homology analysis. Result] The mortality of second instar of Anoplophora glabripennis(Motsch.) reached 72.7% 8 d after 1010 cfu/ml BH-1 was inoculated. The homology of 16S DNA sequences between BH-1 and Serratia marcescens accessed in GenBank reached 99.5%. Combined with the results of routine identification,BH-1 was identified as S. marcescens. Conclusion] BH-1 could be used for biological control of Anoplophora glabripennis (Motsch.). |
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| Keywords: | Anoplophora glabripennis (Motsch ) Serratia marcescens 16S rDNA Phylogeny |
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