| 猪繁殖与呼吸综合症GP5蛋白的纯化与免疫活性分析(英文) |
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| 引用本文： | 陈妍,田宏,尹双辉,尚佑军,刘湘涛.猪繁殖与呼吸综合症GP5蛋白的纯化与免疫活性分析(英文)[J].Agricultural Science&Technology,2009(3). |
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| 作者姓名： | 陈妍 田宏 尹双辉 尚佑军 刘湘涛 |
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| 作者单位： | 中国农业科学院兰州兽医研究所;家畜疫病病原生物学国家重点实验室;农业部畜禽病毒学重点开放实验室; |
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| 基金项目： | Supported by National High-tech Research and Development Plan (863 Project) in the Eleventh Five-Year Plan Period (2006AA10A207)~~ |
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| 摘 要： | Objective] The purification and immunocompetence of GPS protein in porcine reproductive and respiratory syndrome (PRRS) were analyzed in this study, which provided basis for establishing the corresponding serological method. Method] The recombinant expression plasmid pGEX-6P-5 was transformed into BL21 and expressed after being induced with IPTG. The solubility analysis of expression products was carried out, and then the recombinant protein was purified for SDS-PAGE identification and Western-blot analysis. Finally, the recombinant antigen was used in the immune experiment of guinea pigs. Result] The target protein content accounted for 30% of the total cells protein content according to the chromatography scanning, and the purity of target protein after purification reached 80%. The purified protein was analyzed by Western-blot and immune experiment of guinea pigs, and the results showed that the expressed protein had good reactionogenicity and immunogenicity. Conclusion] This study provides materials for further studies on the function between PRRSV ORF5 gene and its editing protein, which also lays a foundation for porcine reproductive and respiratory syndrome virus genetic engineering products.
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| 关 键 词： | 硝酸纤维素膜 可溶性分析 豚鼠 蛋白纯化 表达产物 免疫 弗氏不完全佐剂 猪繁殖与呼吸综合症 弗氏完全佐剂 大肠杆菌 |
Purification and Immunocompetence Analysis of GP5 Protein in Porcine Reproductive and Respiratory Syndrome |
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| CHEN Yan,TIAN Hong,YIN Shuang-hui,SHANG You-jun,LIU Xiang-tao Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Lanzhou.Purification and Immunocompetence Analysis of GP5 Protein in Porcine Reproductive and Respiratory Syndrome[J].农业科学与技术(英文版),2009(3). |
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| Authors: | CHEN Yan TIAN Hong YIN Shuang-hui SHANG You-jun LIU Xiang-tao Key Laboratory of Animal Virology of Ministry of Agriculture State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences Lanzhou |
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| Institution: | CHEN Yan,TIAN Hong,YIN Shuang-hui,SHANG You-jun,LIU Xiang-tao Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Lanzhou 730046 |
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| Abstract: | Objective] The purification and immunocompetence of GPS protein in porcine reproductive and respiratory syndrome (PRRS) were analyzed in this study, which provided basis for establishing the corresponding serological method. Method] The recombinant expression plasmid pGEX-6P-5 was transformed into BL21 and expressed after being induced with IPTG. The solubility analysis of expression products was carried out, and then the recombinant protein was purified for SDS-PAGE identification and Western-blot analys... |
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| Keywords: | Porcine reproductive and respiratory syndrome GP5 Expression Purification Immunocompetence |
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