| 马MxA基因第12外显子多态性检测(英文) |
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| 引用本文： | 菊林花,金花,呼都特.马MxA基因第12外显子多态性检测(英文)[J].Agricultural Science&Technology,2009(1). |
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| 作者姓名： | 菊林花 金花 呼都特 |
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| 作者单位： | 内蒙古农业大学动物科学与医学学院;内蒙古锡林郭勒职业学院医学系; |
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| 基金项目： | Supported by the Natural Science Foundation of Inner Mongolia Au-tonomous Region (200508010413)~~ |
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| 摘 要： | Objective] The study aimed to establish a fast and accurate method to detect the polymorphism of the 12th exon of equine MxA gene. Method] The 12th exon of MxA gene was amplified by mismatch PCR and the products were analyzed by restriction fragment length polymorphism (RFLP) to determine the point mutation at the 1 790 nt of MxA cDNA. The sequence of the PCR products was also analyzed. Result] There were three genotypes (AA, AB and BB) in the 12th exon of equine MxA gene; the 2 081 nt of MxA cDNA mutated from G to C, correspondingly changing the 562th amino acid of the coding region of MxA protein from tryptophan to cysteine; the specific sequence of the PCR products amplified by mismatch PCR-RFLP was consistent with the analysis results of RFLP. Conclusion] The mismatch PCR-RFLP was an easy method with accurate results to detect the polymorphism of the 12th exon of equine MxA gene.
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| 关 键 词： | 核苷酸序列 限制性内切酶 酶切位点 外显子 扩增产物 蛋白基因 酶切图谱 引物 限制性片断长度多态性 多态位点 |
Polymorphism Detection of the Twelfth Exon of Equine MxA Gene |
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| JU Lin-hua,JIN Hua,HU Du-te. College of Animal Science , Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot ,. Medical.Polymorphism Detection of the Twelfth Exon of Equine MxA Gene[J].农业科学与技术(英文版),2009(1). |
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| Authors: | JU Lin-hua JIN Hua HU Du-te College of Animal Science Veterinary Medicine Inner Mongolia Agricultural University Hohhot Medical |
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| Institution: | JU Lin-hua1,JIN Hua2,HU Du-te11. College of Animal Science , Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,2. Medical Department,Xilingol Vocational College,Xilinhot 026000 |
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| Abstract: | Objective] The study aimed to establish a fast and accurate method to detect the polymorphism of the 12th exon of equine MxA gene. Method] The 12th exon of MxA gene was amplified by mismatch PCR and the products were analyzed by restriction fragment length polymorphism (RFLP) to determine the point mutation at the 1 790 nt of MxA cDNA. The sequence of the PCR products was also analyzed. Result] There were three genotypes (AA, AB and BB) in the 12th exon of equine MxA gene; the 2 081 nt of MxA cDNA mutate... |
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| Keywords: | Horse MxA gene Polymorphism |
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