| 植物HSP70反义基因工程雄性不育表达载体的构建及鉴定(英文) |
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| 作者单位： | 中国科学院遗传发育所，中国科学院遗传发育所，中国科学院遗传发育所，中国科学院遗传发育所，中国科学院遗传发育所，聊城大学生命科学学院，聊城大学生命科学学院，聊城大学生命科学学院 北京100101 聊城大学生命科学学院，山东聊城252059，北京100101，北京100101，北京100101，北京100101，山东聊城252059，山东聊城252059，山东聊城252059 |
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| 摘 要： | Objective]In order to study the relation between the HSP70 gene and male sterility of plant further.Method]s]Anther specific expression promoter Osg6B of rice was coloned by PCR then connected with HSP70 antisense fragment to construct HSP70 antisense expression vector.The expression vector was identified by PCR experiment and enzyme digestion.Result]The sequence of coloned Osg6B promoter had 97% homology to the published sequence,and the cis-regulatory element in promoter area was integrated.HSP70 antisense expression vector driven by the promoter Osg6B was confired by colony PCR and enzyme digestion.Conclusion]The construction of expression vector would lay solid foundation for utilization of genetic engineering male sterility of plant.
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Construction and Identification of HSP70 Antisense RNA Expression Vector for Genetic Engineering Male Sterility in Plant |
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| Abstract: | Objective]In order to study the relation between the HSP70 gene and male sterility of plant further.Methods]Anther specific expression promoter Osg6B of rice was coloned by PCR then connected with HSP70 antisense fragment to construct HSP70 antisense expression vector.The expression vector was identified by PCR experiment and enzyme digestion.Result]The sequence of coloned Osg6B promoter had 97% homology to the published sequence,and the cis-regulatory element in promoter area was integrated.HSP70 antisense expression vector driven by the promoter Osg6B was confired by colony PCR and enzyme digestion.Conclusion]The construction of expression vector would lay solid foundation for utilization of genetic engineering male sterility of plant. |
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| Keywords: | HSP70 Osg6B promoter Gene engineering Male sterility |
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