| 基于体细胞组织培养的甘蓝型油菜核三系保持系绵7MB-1亲本繁殖与F_1制种(英文) |
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| 引用本文： | 蒙大庆,袁代斌,张跃非,郭子荣,胥岚,李芝凡,蒲定福,汤天泽,贺启川,陈军.基于体细胞组织培养的甘蓝型油菜核三系保持系绵7MB-1亲本繁殖与F_1制种(英文)[J].Agricultural Science&Technology,2009(1). |
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| 作者姓名： | 蒙大庆 袁代斌 张跃非 郭子荣 胥岚 李芝凡 蒲定福 汤天泽 贺启川 陈军 |
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| 作者单位： | 四川绵阳市农业科学研究所; |
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| 基金项目： | Supported by "11thFive-Year" Crop Breeding Research of SichuanProvince ;;"11thFive-Year" Joint Breeding Research Project Fun-ding of Sichuan Province.~~ |
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| 摘 要： | Objective] The aim was to study the reproduction of the three-line genic male sterile (GMS) line parent Mian 7MB-1 (B.Napus L.) and the seed production of F1 through somatic tissue culture. Method] Through hybridization, a new breeding material Mian 7MB-1 in three-line genic temporary maintainer line propagated by tissue culture was used to improve the sterile plant rate of rapeseed in dual-purpose recessive GMS line, such as Mian 7AB type, S45AB type, and etc. And then the variety comparative test was performed. Result] In order to avoid some fertility restoration phenomena occurring during the process of self-reproduction, Mian 7AB was propagated in bulk with somatic tissue culture of temporary maintainer line plant stem. The propagated temporary maintainer line seedlings were applied to the breeding and seed production of net room male sterile line parent, promoting the sterile plant rate of the male sterile line parent to 91.7%-93.5%. The male sterile line parents per hectare were enough for the seed production of hybrid F1 in 7 500-15 000 hm2. Conclusion] Compared with the original dual-purpose GMS line, the seed production ultilizing male sterile line with high sterile plant rate greatly reduced the labor, significantly improved the seed yield, ensuring the seed quality and forming a perfect breeding and seed production system.
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| 关 键 词： | 三系杂交 三系不育系 临保系 不育株率 制种产量 两系不育系 农艺性状 可育株 体细胞组织培养 两系杂交 |
Reproduction of the Three-line Genic Male Sterile Line Parent Mian 7MB-1 (Brasscia Napus L.) and Seed Production of F_1 Based on Somatic Tissue Culture |
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| MENG Da-qing,YUAN Dai-bin,ZHANG Yue-fei,GUO Zi-rong,XU Lan,LI Zhi-fan,PU Ding-fu,TANG Tian-ze,HE Qi-chuan,CHEN JunMianyang Institute of Agricultural Sciences,Mianyang.Reproduction of the Three-line Genic Male Sterile Line Parent Mian 7MB-1 (Brasscia Napus L.) and Seed Production of F_1 Based on Somatic Tissue Culture[J].农业科学与技术(英文版),2009(1). |
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| Authors: | MENG Da-qing YUAN Dai-bin ZHANG Yue-fei GUO Zi-rong XU Lan LI Zhi-fan PU Ding-fu TANG Tian-ze HE Qi-chuan CHEN JunMianyang Institute of Agricultural Sciences Mianyang |
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| Institution: | MENG Da-qing,YUAN Dai-bin,ZHANG Yue-fei,GUO Zi-rong,XU Lan,LI Zhi-fan,PU Ding-fu,TANG Tian-ze,HE Qi-chuan,CHEN JunMianyang Institute of Agricultural Sciences,Mianyang 621023 |
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| Abstract: | Objective] The aim was to study the reproduction of the three-line genic male sterile (GMS) line parent Mian 7MB-1 (B.Napus L.) and the seed production of F1 through somatic tissue culture. Method] Through hybridization, a new breeding material Mian 7MB-1 in three-line genic temporary maintainer line propagated by tissue culture was used to improve the sterile plant rate of rapeseed in dual-purpose recessive GMS line, such as Mian 7AB type, S45AB type, and etc. And then the variety comparative test was pe... |
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| Keywords: | B Napus L Tissue culture Three-line GMS Breeding and seed production |
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