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绿头鸭线粒体ND2基因全序列测定及其研究(英文)
引用本文:涂剑锋,司方方,邢秀梅,杨福合.绿头鸭线粒体ND2基因全序列测定及其研究(英文)[J].Agricultural Science&Technology,2009(3).
作者姓名:涂剑锋  司方方  邢秀梅  杨福合
作者单位:中国农业科学特产研究所;
基金项目:Supported by National Key Technology R&D program(2006BAD06B06);;National Infrastructure of Natural Resources for Science and Technology(2004DKA30460)~~
摘    要:Objective] The study was to analyze the phylogenesis of Anas platyrhynchos. Method] Complete sequence of mitochondrial ND2 gene of 4 Anas platyrhynchos was determined by direct DNA sequencing based on PCR products. Combined with ND2 gene sequences of the Anas Linnaeus accessed in GenBank, phylogenetic tree was constructed by Neighbor-joining and maximum parsimony methods. Result] The ND2 gene sequences of 4 Anas platyrhynchos were identical(1 041 bp in length; the nucleotide contents of A, G, T, and C were 28.91%, 13.35%, 20.75% and 36.98% respectively; A+T content approximated to that of C+G). Sequences of ND2 gene of mallard were same as spotbill duck, and had high homology with others. The phylogenetic trees indicated mallard and spotbilled duck were close in genetic relationship, both shared a haplotype; then Philippine duck, green-winged teal and northern pintail fell into branch 'A". Conclusion] The domestic duck may be domesticated from mallard and spotbilled duck.

关 键 词:线粒体  系统进化关系  基因序列  遗传距离  斑嘴鸭  绿头鸭  系统进化树  赤颈鸭  

Determination and Analysis of Mitochondrial ND2 Gene Sequence of Anas platyrhynchos
TU Jian-feng,SI Fang-fang,XING Xiu-mei,YANG Fu-he Institute of Special Economic Animal , Plant of Science,Chinese Academy of Agricultural Science,Jilin.Determination and Analysis of Mitochondrial ND2 Gene Sequence of Anas platyrhynchos[J].农业科学与技术(英文版),2009(3).
Authors:TU Jian-feng  SI Fang-fang  XING Xiu-mei  YANG Fu-he Institute of Special Economic Animal  Plant of Science  Chinese Academy of Agricultural Science  Jilin
Institution:TU Jian-feng,SI Fang-fang,XING Xiu-mei,YANG Fu-he Institute of Special Economic Animal , Plant of Science,Chinese Academy of Agricultural Science,Jilin 132109
Abstract:Objective] The study was to analyze the phylogenesis of Anas platyrhynchos. Method] Complete sequence of mitochondrial ND2 gene of 4 Anas platyrhynchos was determined by direct DNA sequencing based on PCR products. Combined with ND2 gene sequences of the Anas Linnaeus accessed in GenBank, phylogenetic tree was constructed by Neighbor-joining and maximum parsimony methods. Result] The ND2 gene sequences of 4 Anas platyrhynchos were identical(1 041 bp in length; the nucleotide contents of A, G, T, and C we...
Keywords:Anas platyrhynchos  ND2 gene  sequence analysis  Phylogenetic tree  
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