冬凤兰非共生萌发和低温离体保存(英文) |
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作者单位: | LUO Yuan-hua1,LENG Qing-yun1,MO Rao1,CHEN Ye-yuan2 1.College of Agronomy,South China University of Tropical Agriculture,Danzhou 571737;2.Tropical Crop Genetic Resource Research Institute,Chinese Academy of Tropical Agriculture Science,Danzhou 571737 |
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摘 要: | Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro.Method] With mature seeds of C.dayanum as explants,different media were selected to establish asymbiotic germination technique system.With protocorms as materials,conservation,resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily.Result] Mature seeds of C.dayanum could germinate after cultured 90 days on MS media as well as "Hyponex 1" media.The germination rate reached more than 98%.Protocorms inoculated in "Hyponex 1" media could be conserved continuously at 5 ℃ in dark for more than 18 months and the survival rate could reach 90%.Conserved protocorms could realize resumptive proliferation culture both on 1/2 MS and "Hyponex 1" media.The seedling-strengthening and rooting media were 1/2 MS media.Conclusion] This research provided practical basis for in vitro conservation and rapid propagation of C.dayanum germplasm resource.
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Asymbiotic Germination and Low-temperature in Vitro Conservation of Cymbidium Dayanum |
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Authors: | LUO Yuan-hua LENG Qing-yun MO Rao CHEN Ye-yuan College of Agronomy South China University of Tropical Agriculture Danzhou Tropical Crop Genetic Resource Research Institute Chinese Academy of Tropical Agriculture Science Danzhou |
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Institution: | LUO Yuan-hua1,LENG Qing-yun1,MO Rao1,CHEN Ye-yuan2 1.College of Agronomy,South China University of Tropical Agriculture,Danzhou 571737,2.Tropical Crop Genetic Resource Research Institute,Chinese Academy of Tropical Agriculture Science,Danzhou 571737 |
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Abstract: | Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro.Method] With mature seeds of C.dayanum as explants,different media were selected to establish asymbiotic germination technique system.With protocorms as materials,conservation,resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily.Result] Mature seeds of C.dayanum could germinate after cultured 90 days on MS media as well as "Hyponex 1" media.The germination rate reached more than 98%.Protocorms inoculated in "Hyponex 1" media could be conserved continuously at 5 ℃ in dark for more than 18 months and the survival rate could reach 90%.Conserved protocorms could realize resumptive proliferation culture both on 1/2 MS and "Hyponex 1" media.The seedling-strengthening and rooting media were 1/2 MS media.Conclusion] This research provided practical basis for in vitro conservation and rapid propagation of C.dayanum germplasm resource. |
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Keywords: | Cymbidium dayanum Asymbiotic germination Protocorm Low-temperature in vitro conservation |
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