Characterization of MicroRNA* Species in Peking Duck Skin |
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| Authors: | Li ZHANG Xiu-juan XIE Shan-gang JIA Shu-dai LIN Li-long AN Wen LUO Xin-zheng JIA Qing-hua NIE Xi-quan ZHANG |
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| Institution: | 1. Agricultural College, Guangdong Ocean University, Zhanjiang 524088, P.R. China;2. Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R. China;3. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, P.R. China |
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| Abstract: | A substantial fraction of miRNA* species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA* species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA* species in two skin small RNA libraries and identified 56 miRNA/miRNA* (miR/miR*) pairs. Nucleotide bias of miRNA* indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3′-U accounted for a higher proportion in the 56 miR/miR* pairs. Among the top 20 expressed miRNA* species, 17 were shared by two libraries and most of the miRNA* species were highly conservative, especially in the “seed region”. miR-199a* were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA* species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels. |
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| Keywords: | Peking duck miRNA star species (miRNA*) skin nucleotide bias |
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