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重组干酪乳杆菌代谢产物抑菌效果的研究
引用本文:谭君,侯喜林,余丽芸,李剑华,王桂华,曹宁.重组干酪乳杆菌代谢产物抑菌效果的研究[J].黑龙江八一农垦大学学报,2012,24(4):29-32.
作者姓名:谭君  侯喜林  余丽芸  李剑华  王桂华  曹宁
作者单位:1. 黑龙江八一农垦大学动物科技学院,大庆,163319
2. 黑龙江八一农垦大学动物科技学院,大庆163319 黑龙江八一农垦大学生命科学技术学院
3. 大庆油田总医院
基金项目:黑龙江省农垦总局攻关项目
摘    要:试验对重组干酪乳杆菌代谢产物的抑菌效果进行研究。利用牛津杯法研究了干酪乳杆菌代谢产物(不同pH值、热和胃蛋白酶处理)对大肠杆菌、金黄色葡萄球菌抑制效果。结果表明乳杆菌的代谢产物对大肠杆菌、金黄色葡萄球菌均有明显抑制作用,在pH值2.0~4.0时有抑菌活性,并且代谢产物对热稳定,其活性不被蛋白酶破坏。结论:重组干酪乳杆菌能产生对肠道致病菌具有抑制作用的代谢产物。

关 键 词:干酪乳杆菌  牛津杯  抑菌

Study on the Bacteriostasis Property of Recombinant Lactobacillus casei Metabolites
Tan Jun,Hou Xilin,Yu Liyun,Li Jianhua,Wang Guihua,Cao Ning.Study on the Bacteriostasis Property of Recombinant Lactobacillus casei Metabolites[J].Journal of Heilongjiang August First Land Reclamation University,2012,24(4):29-32.
Authors:Tan Jun  Hou Xilin  Yu Liyun  Li Jianhua  Wang Guihua  Cao Ning
Institution:1(1.College of Animal Science and Veterinary Madicine,Heilongjiang Bayi Agricultural University,Daqing 163319; 2.College of Life Science and Technology,Heilongjiang Bayi Agricultural University;3.Daqing Oilfield General Hospital)
Abstract:The bacteriostasis of recombinant L.casei metabolites was studied.The oxford plate assay system was used to detect the bacteriostasis of the metabolites on E.coli and S.aureus under treatment of different pH values,heating,and pepsin.The results showed that the metabolites were active in pH 2.0~4.0,heat stable and constant with pepsin.Conclusion: the metabolites of recombinant L.casei had bacteriostasis effection on some pathogenic bacteria.
Keywords:lactobacillus casei  oxford plate  bacteriostasis
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