| 狭卵链格孢菌株AAEC05-3产Tenuazonic acid条件的优化 |
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| 引用本文： | 姜述君,石园园,杨多坤,范文艳,朱丹,戴凌燕,王艳红.狭卵链格孢菌株AAEC05-3产Tenuazonic acid条件的优化[J].黑龙江八一农垦大学学报,2009,21(5):1-4,9. |
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| 作者姓名： | 姜述君 石园园 杨多坤 范文艳 朱丹 戴凌燕 王艳红 |
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| 作者单位： | 黑龙江八一农垦大学,大庆,163319 |
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| 基金项目： | 黑龙江省科技攻关项目，黑龙江八一农垦大学博士启动基金 |
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| 摘 要： | 利用单因素试验和正交设计试验,对微生物除草剂狭卵链格孢菌株AAEC05-3产Tenuazonic acid（TeA）发酵条件进行了研究。研究结果表明,乙酸钠和发酵初始pH值对菌株产TeA影响最大。最佳发酵培养基为：麸皮15g·L^-1、玉米粉15g·L^-1、豆粕15g·L^-1、尿素2g·L^-1、乙酸钠2g·L^-1、氯化钾0.5g·L^-1、磷酸氢二钾2g·L^-1、硫酸镁0.5g·L^-1、硫酸亚铁0.01g·L^-1。最适宜的产tenuazonic acid的发酵工艺条件是初始pH为6,温度为28℃,发酵周期为7d,摇床转速为120r·min^-1。
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| 关 键 词： | 狭卵链格孢菌株 发酵条件 Tenuazonic acid 微生物除草剂 |
The Optimal Condition of Producing Tenuazonic Acid by Alternaria augustiovoidea strain AAEC05-3 |
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| Jiang Shujun,Shi Yuanyuan,Yang Duokun,Fan Wenyan,Zhu dan,Dai Lingyan,Wang Yanhong.The Optimal Condition of Producing Tenuazonic Acid by Alternaria augustiovoidea strain AAEC05-3[J].Journal of Heilongjiang August First Land Reclamation University,2009,21(5):1-4,9. |
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| Authors: | Jiang Shujun Shi Yuanyuan Yang Duokun Fan Wenyan Zhu dan Dai Lingyan Wang Yanhong |
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| Institution: | Jiang Shujun,Shi Yuanyuan,Yang Duokun,Fan Wenyan,Zhu dan,Dai Lingyan,Wang Yanhong(Heilongjiang Bayi Agricultual University,Daqing 163319) |
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| Abstract: | The fermentation conditions for producing tenuazonic acid （TEA） by Alternaria augustiovoidea AAEC05-3 were investigated through single factor and orthogonal design test. The results showed that the main factors affecting TeA production we＇re sodium acetate and initial pH among the orthogonal factors.The optimal fermentation media composition for producing TeA were brans 15 g·L^-1, corn meal 15g·L^-1,bean pulp 15 g·L^-1, urea 2 g·L^-1,CH3COONa 2 g·L^-1, KCI 0.5g·L^-1, K2HPO4 2g·L^-1, MgSO4·TH2O 0.5g·L^-1,FeSO4·7H2O 0.01g·L^-1. The optimum process conditions were a initial pH value of 6, a temperature of 28℃, fermentation time of 7 days, rotation speed of 120 r·min^-1. |
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| Keywords: | Tenuazonic acid |
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