| 三江白猪GH基因多态性分析及对仔猪初生重的影响 |
| |
| 引用本文： | 金甲正,符亚原,黄大鹏,张虎.三江白猪GH基因多态性分析及对仔猪初生重的影响[J].黑龙江八一农垦大学学报,2011,23(3):30-33. |
| |
| 作者姓名： | 金甲正 符亚原 黄大鹏 张虎 |
| |
| 作者单位： | 黑龙江八一农垦大学动物科技学院,大庆,163319 |
| |
| 基金项目： | 黑龙江八一农垦大学博十启动基金 |
| |
| 摘 要： | 为研究三江白猪GH基因多态性对仔猪初生重的影响,试验采用PCR-RFLP方法,对三江白猪GH基因+486到+669区域共183 bp片段进行扩增,使用Dde I限制性内切酶对扩增片段进行多态性检测.结果显示:对扩增产物使用Dde I酶切后产生了3种基因型(AA、AB和BB),AB基因型的仔猪初生重显著高于AA、BB基因...
|
| 关 键 词： | 三江白猪 GH基因 多态性 PCR-RFLP 初生重 |
Polymorphism of the Growth Hormone Gene and its Association with the Birth Weight in Sanjiang White Pigs |
| |
| Jin Jiazheng,Fu Yayuan,Huang Dapeng,Zhang Hu.Polymorphism of the Growth Hormone Gene and its Association with the Birth Weight in Sanjiang White Pigs[J].Journal of Heilongjiang August First Land Reclamation University,2011,23(3):30-33. |
| |
| Authors: | Jin Jiazheng Fu Yayuan Huang Dapeng Zhang Hu |
| |
| Institution: | (College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319) |
| |
| Abstract: | To study the effect of polymorphism of GH gene in Sanjiang white pigs on birth weight,the PCR-RFLP method is used to expand the total 183 bp segments from GH gene ＋486 to ＋669 region for Sanjiang White Pigs,and DdeⅠ is used to exam the polymorphisms for expand segment.The results showed that there are three genotypes（AA,AB,BB） when the PCR products were cut by DdeⅠ,the birth weight of the individuals with AB genotype were higher than the ones with genotype of AA and BB（P0.05）,and the difference is not significant between the genotype AA and BB（P0.05）. |
| |
| Keywords: | PCR-RFLP |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
