| O型口蹄疫病毒VP1-2A基因及多表位片段在毕赤酵母中的表达 |
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| 引用本文： | 袭莹,朱战波,金宁一,胡博,任静强,刘存霞,张军,王鹤,鲁会军.O型口蹄疫病毒VP1-2A基因及多表位片段在毕赤酵母中的表达[J].黑龙江八一农垦大学学报,2010,22(5):60-63. |
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| 作者姓名： | 袭莹 朱战波 金宁一 胡博 任静强 刘存霞 张军 王鹤 鲁会军 |
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| 作者单位： | [1]黑龙江八一农垦大学动物科技学院,大庆163319 [2]军事医学科学院军事兽医研究所 全军基因工程重点实验室 ,大庆163319 [3]吉林大学畜牧兽医学院 ,大庆163319 [4]吉林农业大学动物科技学院,大庆163319 |
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| 基金项目： | 国家"863"计划重点项目 |
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| 摘 要： | 利用毕赤酵母表达系统串联表达O型口蹄疫病毒(FMDV)VP1-2A基因及O型FMDV多表位片段(CTE),将O型FMDVvp1基因和CTE多表位片段克隆到毕赤酵母分泌性表达载体pPIC9K中,构建重组表达载体pPIC9K-VP1-2A-CTE并测序。经SalI线性化后,通过电击转化法将重组质粒导入毕赤酵母GS115中,并对表达产物用SDS-PAGE和Western blot进行分析。重组质粒通过电转进入毕赤酵母后,能表达相对分子量为41.8KD(CTE)和26.5KD(VP1-2A)的蛋白,经Western blot分析,两种蛋白均具有抗原性。在毕赤酵母中成功地表达O型FMDVVP1-2A蛋白和多表位片段(CTE),为研制新型口蹄疫的基因工程疫苗奠定了基础。
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| 关 键 词： | 口蹄疫 VP1-2A基因 多表位片段（CTE） 毕赤酵母 分泌表达 |
Expression of Recombinant Pichia pastoris VP1-2A and Multi-epitopes of Type O Foot-and-Mouth Disease Virus |
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| Xi Ying,Zhu Zhanbo,Jin Ningyi,Hu Bo,Ren Jingqiang,Liu Cunxia,Zhang Jun,Wang He,Lu Huijun.Expression of Recombinant Pichia pastoris VP1-2A and Multi-epitopes of Type O Foot-and-Mouth Disease Virus[J].Journal of Heilongjiang August First Land Reclamation University,2010,22(5):60-63. |
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| Authors: | Xi Ying Zhu Zhanbo Jin Ningyi Hu Bo Ren Jingqiang Liu Cunxia Zhang Jun Wang He Lu Huijun |
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| Institution: | 1.College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319; 2. Genetic Engineering Laboratory of PLA,Academy of Military Medical Sciences of PLA;3.College of Animal Science and Veterinary Medicine,Jilin University;4. College of Animal Science and Technology,Jilin Agricultural University) |
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| Abstract: | To express and identify O-type foot and mouth disease virus protein VP1-2A and CTE epitope fagment in yeast Pichia pastoris.FMDV VP1-2A gene and CTE epitope fagment were cloned into secretory Pichia pastoris expression vector-pPIC9K and sequenced. After being linearized with Sal I enzyme digestion,the vector was transformed into Pichia pastoris GS115 by electroporation. Expressed proteins in yeast were analyzed by SDS-PAGE and Western blot. The results of SDS-PAGE and Western blot demonstrated that the culture supernatant of recombinant yeast contained VP1-2A and CTE protein. FMDV VP1-2A and CTE epitope fagment were expressed successfully in yeast Pichia pastoris,and had good immunogenicity which lays the foundation for further FMDV vaccine research. |
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| Keywords: | FMDV VP1-2A CTE pichia pastoris immunogenicity |
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